Search

Objective: Based on the quality analysis of Nauclea officinalis in different growth years and different months in the same year, the most suitable harvest year and the specific month in the same year were determined according to the evaluation of the active components in the stem of medicinal parts of Nauclea officinalis. Methods: LC-MS/MS method was used to investigate the quality of Nauclea officinalis from different growth years and different months in the same year, mainly including the content determination of the active ingredients in the medicine. Results: With the prolongation of production life, the content of active ingredients in stem of Nauclea officinalis increased continuously. After reaching a certain number of years, the content of active ingredients increased little;In the middle of the same year, from mid-October to mid- June of the next year, the content of iso-vincin lactam was relatively high, especially between the end of March and the end of May. The content was high and stable, and the highest point was the stem sample collected in May. Conclusion: The content of each component in the stem of Nauclea officinalis with a growth life of more than 5 years has reached a relatively high and stable level.Nauclea officinalis with a growth life of more than 5 years should be collected as raw materials for medicinal materials. The month with high and stable content of isocyanoside lactam, namely May of each year, was taken as the best harvest time of gallbladder in one year. The best harvest time was from mid-October to mid-June of the next year, or from March to May of each year.

Objective: To study the effect of the extracts from Callicarpa nudiflora on wound healing and the preparation of ointment. Methods: Totally 50 KM mice were randomly divided into five groups, which were negative control group ,positive control group, high, middle and low dose groups of Callicarpa nudiflora ointment. Mice ear swelling test was conducted to investigate the effect of Callicarpa nudiflora induced by xylene, Bacteriostasis experiments were conducted to observe the bacteriostasis effects of Callicarpa nudiflora on five indicator bacteria, i.e. Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Bacillus subtilis, and Staphylococcus aureus in vitro. A total of 30 new zealand white rabbit with half male and half female were randomly divided into five groups, which were negative control group, positive control group, high, middle and low groups of Callicarpa nudiflora ointment. The rabbits were treated with sterilizing blade and treated with external medicine to observe the effect of the extracts from Callicarpa nudiflora on wound healing. Orthogonal test was used to determine the preparation method of Callicarpa nudiflora ointment， Physical properties, centrifugal stratification test, heat and cold resistance test were conducted. Results: Bacteriostasis experiments in vitro showed high dose group of Callicarpa nudiflora extracts got 16.8 cm antimicrobial zone of Pseudomonas aeruginosa. The experimental results showed that the ear swelling degree of mice induced by xylene was significantly reduced in the middle and high dose groups. All the treatment groups could promote skin healing of rabbit significantly compared with negative control group. There were significantly differences between all the treatment groups and negative control group on the wound healing rate. The optimum preparation process was determined by orthogonal screening test. The optimal formula was as follows： Twain-80 1.2 g, glycerin monostearate 4.7 g, glycerinum 0.9 g, liquid paraffin 10.8 g, Span80 1.4 g， solid paraffin 1.4 g， vaseline 0.9 g. The emulsification temperature was 65 ℃,and emulsification time was 25 min. Conclusions: The extract of Callicarpa nudiflora can be used as external preparation and the preparation is in compliance with Chinese pharmacopoeia 2015.

Objective: To study the antioxidant activities of five extracts of Amomi longiligulare (A. longiligulare) to determine the antioxidant fraction. Methods: The ethanol extract of A. longiligulare was extracted by systematic solvent extraction to obtain the petroleum ether, dichloromethane, ethyl acetate, n-butanol fractions successively. The in vitro antioxidant activities of the five extracts were evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical and hydroxyl radical (OH-) scavenging assay methods. Results: The five extracts showed different extent of antioxidant activities in vitro, especially the ethyl acetate and dichloromethane fractions. Conclusion: The difference of antioxidant activity of different extracts may be associated with the type and structure of antioxidant components in each fraction of A. longiligulare fruits.

Objective: To explore the effect of Danshen injection on the vascular endothelial function and renal function in patients with pregnancy induced hypertension syndrome (PIH). Methods: A total of 100 patients with PIH who were admitted in our hospital from May, 2015 to May, 2016 were included in the study and randomized into the observation group and the control group. The patients in the control group were given blood pressure reduction, diuresis, spasmolysis, sedation, magnesium sulfate, and comprehensive nursing intervention. On this basis, the patients in the observation group were given additional Danshen injection (20 mL) + 5% glucose (250 mL), ivdrip, 1 time/d. After 10 d treatment, the efficacy was evaluated. The peripheral venous blood before and after treatment in the two groups was collected. The radioimmunoassay was used to detect ET-1. ELISA was used to detect Hcy. The immunoturbidimetry was used to detect vWF. The radioimmunoassay was used to detect BUN, Scr, UA, and β2-MG. The standard sphygmomanometer was used to monitor the blood pressure and MAP was calculated. The biuret colorimetry was used to determine 24 h Upro. Results: The reduced degree of ET-1, Hcy, and vWF after treatment in the observation group was significantly superior to that in the control group. The reduced degree of BUN, Scr, UA, and β2-MG after treatment in the observation group was significantly superior to that in the control group. The reduced degree of MPA and 24 h Upro after treatment in the observation group was significantly superior to that in the control group. Conclusions: Routine treatments, comprehensive nursing intervention, and Danshen injection in the treatment of PIH can effectively improve the vascular endothelial function and renal function in order to reduce the blood pressure and alleviate the urine protein.

Objective: To explore the effect of Danshen injection on the oxidative stress and inflammatory cytokines in patients with pregnancy-induced hypertension syndrome (PIH). Methods: A total of 100 patients with PIH who were admitted in our hospital from June, 2015 to June, 2016 were included in the study and randomized into the observation group and the control group. The patients in the control group were given blood pressure reduction, diuresis, spasmolysis, sedation, magnesium sulfate, and comprehensive nursing intervention. On this basis, the patients in the observation group were given additional Danshen injection (20 mL) + 5% glucose (250 mL), iv drip, 1 time/d. After 10-day treatment, the efficacy was evaluated. The peripheral venous blood before and after treatment in the two groups was collected. The nitric acid reduction method was used to detect the plasma NO. TBA method was used to detect MDA. XOD method was used to detect SOD. ELISA was used to detect the serum HMGB1. Immunoturbidimetric assay was used to detect the serum Cys C. The spectrophotometer was used to detect the serum Hcy. HR and MAP were monitored. Results: The improved degree of NO, SOD, and MDA after treatment in the observation group was significantly superior to that in the control group (P