1. Linker mutagenesis in the gene encoding the periplasmic maltose-binding protein of E. coli.
- Author
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Duplay P, Bedouelle H, Szmelcman S, and Hofnung M
- Subjects
- DNA Restriction Enzymes, DNA Transposable Elements, Escherichia coli metabolism, Maltose-Binding Proteins, Plasmids, ATP-Binding Cassette Transporters, Carrier Proteins genetics, Escherichia coli genetics, Escherichia coli Proteins, Genes, Genes, Bacterial, Genetic Linkage, Membrane Proteins genetics, Monosaccharide Transport Proteins, Mutation, Periplasmic Binding Proteins
- Abstract
A plasmid carrying the malE gene, coding for the periplasmic maltose-binding protein of E. coli, was submitted to random mutagenesis by the insertion of a BamHI linker. About 25% of the clones recovered had acquired a BamHI site in the gene malE. Most of the linker insertions were accompanied by small deletions with an average size of 30 base pairs. Among 21 mutants synthesizing a stable maltose binding protein, 8 were still able to grow on maltose. A preliminary analysis of these mutants indicates that certain regions of the protein may not be essential for maltose transport.
- Published
- 1985
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