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Malinda R Schaefer,1 Jessica Holttum,1 Megan Olson,1 Danielle Westenberg,1 Nathan Rubin,2 Mark R Schleiss,3 Jessica Nyholm1 1Department of Obstetrics, Gynecology & Women’s Health, University of Minnesota Medical School, Minneapolis, MN 55455, USA; 2Biostatistics Core, Masonic Cancer Center, University of Minnesota Medical School, Minneapolis, MN 55455, USA; 3Department of Pediatrics, Division of Pediatric Infectious Diseases and Immunology, University of Minnesota Medical School, Minneapolis, MN 55455, USACorrespondence: Malinda R Schaefer Email schaefer.malinda@gmail.comPurpose: Congenital CMV infection can result in serious sequelae in the newborn. The goal of this study was to assess pregnant women’s knowledge and understanding of CMV infection during pregnancy and develop an educational tool about CMV infection to be utilized during prenatal care.Materials and Methods: This is a prospective intervention study that assessed pregnant women’s knowledge before and after receiving an educational handout about CMV infection in pregnancy and the perceived value of this education. Pre- and post-education questionnaires were utilized to assess knowledge. The pre-education questionnaire and CMV educational handout were given at the same clinic visit. The educational handout was given after the pre-education questionnaire had been completed. The post-education questionnaire was given at the next scheduled prenatal clinic appointment and included questions regarding the level of satisfaction with the education and the perceived value of the information. Pregnant women less than 34 weeks of gestation were eligible.Results: A total of 263 women were enrolled, 263 completed the pre-CMV educational questionnaire and 215 women completed both questionnaires. Some women only partially completed the questionnaires and those partial responses have been included. Prior to education, 33% (85/261) of participants had heard of CMV. This increased to 75% (160/214) after education. Participants scored each of the recommended hygiene practices between 1 and 5 (5 is the most acceptable) and each recommended hygiene practice received an average score between 3.8 and 5. 74% (134/180) of participants reported increasing their hygienic practices after education. 96% (180/188) of participants indicated they were satisifedto have received the education. 98% (187/190) thought more women should receive this education during prenatal care.Conclusion: Pregnant women viewed education about CMV favorably and increased the frequency of recommended hygiene practices. Introducing an educational handout to routine prenatal care may be beneficial in increasing awareness of CMV infection in pregnancy.Keywords: congenital CMV, CMV vaccine, CMV pregnancy, CMV education