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Dalia Elebeedy,1 Aml Ghanem,2,3 Marwa El-Sayed,4 Eman Fayad,5 Ola A Abu Ali,6 Amal Alyamani,5 Asmaa Sayed Abdelgeliel7 1College of Biotechnology, Misr University for Science and Technology (MUST), 6th of October City, 12573, Egypt; 2Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, 12573, Egypt; 3School of Biotechnology, Badr University in Cairo, Badr City, Cairo, 11829, Egypt; 4Department of Microbiology and Immunology, Faculty of Medicine, South Valley University, Qena, 83523, Egypt; 5Department of Biotechnology, Faculty of Science, Taif University, Taif, 21944, Saudi Arabia; 6Department of Chemistry, College of Science, Taif University, Taif, 21944, Saudi Arabia; 7Department of Botany & Microbiology, Faculty of Sciences, South Valley University, Qena, 83523, EgyptCorrespondence: Marwa El-Sayed, Department of Microbiology and Immunology, Faculty of Medicine, South Valley University, Qena, 83523, Egypt, Tel +20 1003717916, Fax +20 963216128, Email marwa.elsayed@med.svu.edu.eg; Asmaa Sayed Abdelgeliel, Department of Botany & Microbiology, Faculty of Sciences, South Valley University, Qena, 83523, Egypt, Tel +20 1002973907, Fax +20 963216128, Email asmaa.elgafari@sci.svu.edu.egPurpose: The developed resistance of pathogenic microorganisms towards the currently used antimicrobial agents requires the fast search for newer potent antimicrobials. One of the most important ways to combat the previously mentioned disaster is the use of natural alternatives like medicinal plants. Our study aimed to estimate the anti-inflammatory property, and antibacterial effects of probiotics Lactiplantibacillus plantarum and ethanol extracts of Lawsonia inermis leaves against Staphylococcus aureus when they were used separately or collectively as synergism.Material and Methods: Experimentally induced infected wound model in mice was created and divided into 10 groups then treated for two days by L. plantarum and L. inermis individually or in combination, followed by biochemical assays. The antibacterial, anti-inflammatory, and wound healing activity were evaluated through histopathological sections taken before and after treatment.Results: Our results revealed that L. plantarum and L. inermis mixture could inhibit growth of S. aureus and decrease the minimal inhibitory concentration (MIC) of L. plantarum to 2 mg/mL. The mixture decreased level of both interleukin 6 (IL-6) and interferon-alpha (TNF-α) to a level near the normal uninfected group. Histopathological study showed that animals treated with both L. plantarum and L. inermis had achieved almost 90% healing.Conclusion: These results suggest that L. plantarum and L. inermis mixture has synergistic effect on healing of infected wound.Keywords: Lactiplantibacillus plantarum, Lawsonia inermis, Staphylococcus aureus, wound healing