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Your search keyword '"miR-377"' showing total 2 results
Start Over Descriptor "miR-377" Publisher dove medical press
2 results on '"miR-377"'

1. CircPVT1 Regulates Cell Proliferation, Apoptosis and Glycolysis in Hepatocellular Carcinoma via miR-377/TRIM23 Axis

Author

Bu N, Dong Z, Zhang L, Zhu W, Wei F, and Zheng S

Subjects
hepatocellular carcinoma, circpvt1, mir-377, trim23, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Nan Bu,1,* Zheng Dong,2,* Lingfeng Zhang,3 Weibo Zhu,4 Furong Wei,5 Sheng Zheng6 1Department of Gastroenterology, Chinese Medicine Hospital of Jiamusi City, Heilongjiang Province, Jiamusi, Heilongjiang, People’s Republic of China; 2Department of General Surgery, Ningbo Mingzhou Hospital, Ningbo, Zhejinag, People’s Republic of China; 3Department of Hepatobiliary Surgery, The First People’s Hospital of Huaihua City, Huaihua, Hunan, People’s Republic of China; 4Department of Medical Technology, Qujing Medical College, Qujing, Yunan, People’s Republic of China; 5Department of Gastroenterology, Central Hospital of Haining, Haining, Zhejinag, People’s Republic of China; 6Department of Gastroenterology, The Third People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Sheng ZhengDepartment of Gastroenterology, The Third People’s Hospital of Yunnan Province, 292 Beijing Road, Guandu District, Kunming City, Yunnan Province, People’s Republic of ChinaTel +86-871-63196247Email x5z25a@163.comBackground: Recent studies reported that circular RNAs (circRNAs) exert essential functions in hepatocellular carcinoma (HCC) progression. However, the expression profile and function of circular RNA PVT1 (circPVT1) in HCC are not fully addressed. Thus, we aimed to probe into the function of circPVT1 in HCC development.Methods: The levels of circPVT1, microRNA-377 (miR-377) and transcripts encoding tripartite motif containing 23 (TRIM23) were determined by qRT-PCR. The stability and localization of circPVT1 were examined by RNase R digestion assay and subcellular fraction assay, respectively. Cell proliferation and apoptosis were evaluated by MTT assay and flow cytometry analysis, respectively. The relationship between miR-377 and circPVT1 or TRIM23 was determined by dual-luciferase reporter assay and RNA immunoprecipitation (RIP). The protein expression of TRIM23 was measured by Western blot. The glycolysis level was assessed by specific kits and Seahorse Extracellular Flux Analyzer XF96. The function of circPVT1 in vivo was investigated in a murine xenograft model.Results: CircPVT1 and TRIM23 levels were elevated, while miR-377 was decreased in HCC. CircPVT1 knockdown restrained proliferation and glycolysis, but enhanced apoptosis in HCC cells. CircPVT1 could bind to miR-377 and inhibition of miR-377 restored circPVT1 knockdown-mediated effect on HCC cells. TRIM23 was certified as a target of miR-377, and TRIM23 upregulation overturned the influence of miR-377 upregulation or circPVT1 silence on HCC progression. Moreover, circPVT1 knockdown restrained tumor growth in HCC in vivo.Conclusion: CircPVT1 aggravated the progression of HCC by upregulating TRIM23 via sponging miR-377.Keywords: hepatocellular carcinoma, circPVT1, miR-377, TRIM23

Published
2020

2. Interfering Human Papillomavirus E6/E7 Oncogenes in Cervical Cancer Cells Inhibits the Angiogenesis of Vascular Endothelial Cells via Increasing miR-377 in Cervical Cancer Cell-Derived Microvesicles

Author

Zhang Y, Liu Y, Guo X, Hu Z, and Shi H

Subjects
human papillomavirus e6/e7, lpar2, angiogenesis, mir-377, microvesicle, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282
Abstract

Ying Zhang,1 Yao Liu,2 Xingrong Guo,2 Zhenhua Hu,1 Huirong Shi1 1Department of Gynaecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, People’s Republic of China; 2Department of Gynaecology, Hami Central Hospital, Hami, Xinjiang 839000, People’s Republic of ChinaCorrespondence: Huirong ShiDepartment of Gynaecology, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, 27 District, Zhengzhou City, Henan Province 450052, People’s Republic of ChinaEmail 70219609@qq.comBackground: The dysregulation of the human papillomavirus 18 E6 and E7 oncogenes plays a critical role in the angiogenesis of cervical cancer (CC), including the proliferation, migration, and tube formation of vascular endothelial cells. Interfering E6/E7 increases the number of CC cell-derived microvesicles (CC-MVs). Additionally, microRNAs (miRNAs) can modulate CC angiogenesis and can be encapsulated in MVs.Objective: We aim to investigate whether E6/E7 affects CC angiogenesis via regulating miRNAs in CC-MVs.Methods: CC-MVs were isolated from a CC cell line (HeLa) which were transfected with small interfering RNAs (siRNAs) against E6/E7 or co-transfected with miR-377 mimics/inhibitors. The expression of several miRNAs in CC-MVs was detected using quantitative real-time PCR. After co-incubating CC-MVs with human umbilical vein endothelial cells (HUVECs), cell proliferation, migration, and tube formation of HUVECs were determined using cell counting kit-8, transwell, and tube formation assays, respectively.Results: MiR-377 was increased in E6/E7-interfering CC-MVs. Overexpressing miR-377 in CC-MVs suppressed HUVEC proliferation, migration, and tube formation. LPAR2, the cell surface G protein-coupled receptor, was the downstream target of miR-377 in HUVECs. The co-transfection of E6/E7 siRNAs and miR-377 inhibitors in CCs negated the effect of E6/E7 siRNAs on the elevation of miR-377 in CC-MVs. In HUVECs, the co-transfection of E6/E7 siRNAs and miR-377 inhibitors restored the LPAR2 expression which was reduced by the E6/E7 siRNA transfection. Meanwhile, miR-377 mimic reduced LPAR2 expression and inhibited HUVEC proliferation, migration, and tube formation, while such response was negated by LPAR2 overexpression.Conclusion: Interfering E6/E7 increased miR-377 in CC-MVs, and overexpressing miR-377 in CC-MVs inhibited angiogenesis of HUVECs via reducing LPAR2.Keywords: human papillomavirus E6/E7, microvesicle, miR-377, LPAR2, angiogenesis

Published
2020

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