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Yuriy Prylutskyy,1 Dmytro Nozdrenko,1 Olexandr Omelchuk,2 Svitlana Prylutska,3 Olexandr Motuziuk,1,2 Vasil Soroсa,1 Igor Vareniuk,1 Viktoria Stetska,1 Kateryna Bogutska,1 Uwe Ritter,4 Jacek Piosik5 1ESC ”institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine; 2Faculty of Biology and Forestry, Lesya Ukrainka Volyn National University, Lutsk, Ukraine; 3Faculty of Plant Protection, Biotechnology and Ecology, National University of Life and Environmental Science of Ukraine, Kyiv, Ukraine; 4Institute of Chemistry and Biotechnology, Technical University of Ilmenau, Ilmenau, Germany; 5Intercollegiate Faculty of Biotechnology, University of Gdansk, Gdańsk, PolandCorrespondence: Jacek Piosik, Email jacek.piosik@ug.edu.plIntroduction: Rhabdomyolysis, as an acute stage of myopathy, causes kidney damage. It is known that this pathology is caused by the accumulation of muscle breakdown products and is associated with oxidative stress. Therefore, the present study evaluated the effect of intraperitoneal administration (dose 1 mg/kg) of water-soluble C60 fullerenes, as powerful antioxidants, on the development of rat kidney damage due to rhabdomyolysis caused by mechanical trauma of the muscle soleus of different severity (crush syndrome lasting 1 min under a pressure of 2.5, 3.5, and 4.5 kg/cm2, respectively).Methods: Using tensometry, biochemical and histopathological analyses, the biomechanical parameters of muscle soleus contraction (contraction force and integrated muscle power), biochemical indicators of rat blood (concentrations of creatinine, creatine phosphokinase, urea and hydrogen peroxide, catalase and superoxide dismutase activity), glomerular filtration rate and fractional sodium excretion value, as well as pathohistological and morphometric features of muscle and kidney damages in rats on days 1, 3, 6 and 9 after the initiation of the injury were studied.Results: Positive changes in biomechanical and biochemical parameters were found during the experiment by about 27– 30 ± 2%, as well as a decrease in pathohistological and morphometric features of muscle and kidney damages in rats treated with water-soluble C60 fullerenes.Conclusion: These findings indicate the potential application of water-soluble C60 fullerenes in the treatment of pathological conditions of the muscular system caused by rhabdomyolysis and the associated oxidative stress.Keywords: muscle soleus, С60 fullerene, rhabdomyolysis, biomechanical parameters of muscle contraction, blood biochemical indicators, histopathological analysis

Introduction: Rhabdomyolysis, as an acute stage of myopathy, causes kidney damage. It is known that this pathology is caused by the accumulation of muscle breakdown products and is associated with oxidative stress. Therefore, the present study evaluated the effect of intraperitoneal administration (dose 1 mg/kg) of water-soluble C 60 fullerenes, as powerful antioxidants, on the development of rat kidney damage due to rhabdomyolysis caused by mechanical trauma of the muscle soleus of different severity (crush syndrome lasting 1 min under a pressure of 2.5, 3.5, and 4.5 kg/cm 2 , respectively)., Methods: Using tensometry, biochemical and histopathological analyses, the biomechanical parameters of muscle soleus contraction (contraction force and integrated muscle power), biochemical indicators of rat blood (concentrations of creatinine, creatine phosphokinase, urea and hydrogen peroxide, catalase and superoxide dismutase activity), glomerular filtration rate and fractional sodium excretion value, as well as pathohistological and morphometric features of muscle and kidney damages in rats on days 1, 3, 6 and 9 after the initiation of the injury were studied., Results: Positive changes in biomechanical and biochemical parameters were found during the experiment by about 27-30 ± 2%, as well as a decrease in pathohistological and morphometric features of muscle and kidney damages in rats treated with water-soluble C 60 fullerenes., Conclusion: These findings indicate the potential application of water-soluble C 60 fullerenes in the treatment of pathological conditions of the muscular system caused by rhabdomyolysis and the associated oxidative stress., Competing Interests: The authors declare no conflicts of interest in this work., (© 2024 Prylutskyy et al.)