Search

Eric Priyo Prasetyo,1,2 Mefina Kuntjoro,1,3 Setyabudi Goenharto,2 Devi Eka Juniarti,2 Febriastuti Cahyani,2 Nike Hendrijantini,3 Alexander Patera Nugraha,4 Ninuk Hariyani,5 Fedik Abdul Rantam6,7 1Doctoral Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; 2Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; 3Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; 4Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; 5Department of Dental Public Health, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; 6Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, Indonesia; 7Laboratory of Virology, Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, IndonesiaCorrespondence: Eric Priyo PrasetyoDepartment of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Jalan Mayjend Prof. Dr. Moestopo 47, Surabaya, IndonesiaTel/Fax +62 31 5030255Email eric-p-p@fkg.unair.ac.idPurpose: Calcium hydroxide is a gold standard dental material generally used for pulpal and periapical therapy including regenerative endodontic procedures because of its positive properties. However, evaluation about this material on stem cells is limited. Human umbilical cord mesenchymal stem cells (HUCMSCs) are potential to be used in regenerative therapy. Regenerative therapy needs a sustainable cell supply to maintain its regenerative capacity. The aim of this study was to ascertain the apoptosis result of calcium hydroxide on HUCMSCs through the expression of apoptotic protease-activating factor-1 (APAF-1), caspase-3, and caspase-9.Materials and Methods: This study used a thawed frozen stock of passage 5 HUCMSCs, grown in minimum essential medium (MEM) alpha containing calcium hydroxide at concentration of 0.1 microgram/mL for 1, 3 and 7 days. Polyclonal antibody with fluorescence isothiocyanate (FITC) label was used to evaluate the expressions. APAF-1, caspase-3, and caspase-9 expressions were recorded and compared on every observation day using fluorescence microscope. Analysis of variance was performed to analyze the significance among the results of treatment groups. The results were concluded significant if p< 0.05.Results: The addition of calcium hydroxide in MEM alpha medium increases HUCMSCs expression of APAF-1, caspase-3 and caspase-9 significantly, compared to the control group without calcium hydroxide (p< 0.05) in all the times. Day 1 showed the lowest increase followed by higher expressions on day 3 and day 7.Conclusion: HUCMSCs express increased APAF-1, caspase-3 and caspase-9 after in-vitro calcium hydroxide exposure. This should be considered when using calcium hydroxide on HUCMSCs for regenerative procedures with regard to other positive properties.Keywords: apoptosis, calcium hydroxide, caspases, mesenchymal stem cells, umbilical cord

Taufan Bramantoro,1 Nuraini Indrastie,2 Ninuk Hariyani,1 Dini Setyowati1 1Department of Dental Public Health, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; 2Graduate School of Dental Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaCorrespondence: Taufan BramantoroDepartment of Dental Public Health, Faculty of Dental Medicine Universitas Airlangga, Campus A UNAIR Jl. Prof. Dr. Moestopo No. 47, Surabaya 60132, East Java, IndonesiaTel +62 31 5030255Email taufan-b@fkg.unair.ac.idBackground: Early childhood caries (ECC) is the most common dental caries in children. The 2013 Indonesian basic health survey showed that there were still many children in developmental age affected by dental caries. Parents’ participation is very necessary in guiding, giving understanding, reminding, and providing facilities so that children can maintain their overall oral hygiene. Hence, parents can prevent plaque accumulation and the occurrence of caries in children.Objective: This study aimed to determine the effect of husbands’ support on the decision of mothers to take their children to the dentist.Subjects and methods: This study used a cross-sectional method. The samples from this study were 95 mothers from 3 kindergartens in Surabaya. Ninety-five mothers were randomly chosen as research respondents and filled out the research questionnaires. The data obtained were processed using statistical analysis to determine the correlation.Results: Research respondents with low husband support (subjective norms) who did not take their children to the dentist had a significant value (p = 0.004) compared to the study respondents with high husband support who did not bring their children to the dentist (p = 0.093).Conclusion: Mothers with low support from husbands tend to have the attitude not to take their children to do routine dental and oral examinations.Keywords: children dental care, dental health, health behavior, social support, theory of planned behavior

Ninuk Hariyani,1 Roesanto Heroe Soebekti,1 Dini Setyowati,1 Taufan Bramantoro,1 Listyati Setyo Palupi,2 Oktarina,3 Ekky Putriana41Dental Public Health Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; 2Personality and Social Psychology Department, Faculty of Psychology, Universitas Airlangga, Surabaya, Indonesia; 3Research and Development Centre for Humanities and Health Management, Ministry of Health Republic of Indonesia, Surabaya, Indonesia; 4General Dentist, Private Practice, Surabaya, IndonesiaAim: To assess the prevalence of dental caries among autism spectrum disorders (ASD) children in Surabaya, Indonesia, and to explore the association between oral health-related behaviors comprising toothbrushing, snacking and dental visiting and the severity of caries.Methods: The prevalence and severity of caries were assessed using DMF-T/dmf-t and PUFA/pufa indexes for mixed dentitions. The parents or carers’ responses were collected to identify the oral health-related behaviors that were associated with caries severity.Results: This study shows that almost 79% of ASD children in Surabaya were experiencing caries and 47.1% had untreated caries with pulp involvement or more severe conditions. Oral health behaviour factors associated with the severity of dental caries experienced by children with autism were brushing frequency (B [95% CI] =2.46 [0.09–4.83] and 2.03 [0.78–3.28]), brushing time (B [95% CI] =3.76 [1.32–6.20] and 2.03 [0.74–3.32]) and cleansing after snack (B [95% CI] = 2.04 [0.20–3.89] and 1.21 [0.23–2.18]) in DMF-T/dmf-t and PUFA/pufa indexes, respectively.Conclusions: The prevalence of caries among children with autism in Surabaya was high. The severity was associated with brushing and snacking behaviors. Caries in children with autism should be paid attention, and research in modifying daily activities are needed to allow them to adopt more healthy oral health-related behaviors.Keywords: autism spectrum disorders, caries, oral health related behaviour