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Start Over Descriptor "FOXP4" Publisher dove medical press
2 results on '"FOXP4"'

1. Upregulation of FOXP4 in breast cancer promotes migration and invasion through facilitating EMT

Author

Ma T and Zhang J

Subjects
FOXP4, EMT, snail, migration, breast cancer, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Tao Ma,1–4 Jin Zhang1–4 1The Third Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, P.R. China; 2Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin, P.R. China; 3Key Laboratory of Cancer Prevention and Therapy, Tianjin, P.R. China; 4Tianjin’s Clinical Research Center for Cancer, Tianjin, P.R. China Background: Family of forkhead box transcription factors has been found to play key roles in multiple types of cancer. Materials and methods: Our study is to decipher the effects of FOXP4 in human breast cancer (BC). Quantitative real-time polymerase chain reaction and Western blot analyses were performed to determine the mRNA and protein expressions of FOXP4 in BC tissue samples and cell lines. The gain and loss of function assay were used to explore the detailed roles of FOXP4 in breast cell lines, including MDA-MB-231 and MCF-7 cells. Its effect on BC growth, migration, and invasion were evaluated by colony formation assay, CCK-8 assay, wound-healing assay, and transwell invasion assay, respectively. Results: Our findings revealed that FOXP4 promotes cell proliferation, migration, as well as invasion of BC cells. Furthermore, FOXP4 also facilitates epithelial–mesenchymal transition. ChIP, qChIP assay, and dual luciferase reporter assay were used to examine whether Snail is a downstream target of FOXP4. Moreover, overexpression of Snail could partially rescue the effects of FOXP4 inhibition on cancer cell migration and invasion. Conclusion: Our findings revealed that FOXP4 is a critical regulator in BC. Keywords: FOXP4, EMT, Snail, migration, breast cancer

Published
2019

2. LncRNA SNHG16 Promotes the Progression of Laryngeal Squamous Cell Carcinoma by Mediating miR-877-5p/FOXP4 Axis

Author

Wang X, Liu L, Zhao W, Li Q, Wang G, and Li H

Subjects
mir-877-5p, laryngeal squamous cell carcinoma, foxp4, snhg16, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282
Abstract

Xiaoli Wang,1 Liming Liu,2 Wenfei Zhao,3 Qingyan Li,4 Guangsheng Wang,5 Huahui Li6 1Department of Clinical Laboratory, Jinan City People’s Hospital, Jinan People’s Hospital Affiliated to Shandong First Medical University, Jinan 271199, People’s Republic of China; 2Department of Otorhinolaryngology, Juye County Hospital of TCM, Heze 274900, People’s Republic of China; 3Department of Comprehensive Oncology Therapy, Qingdao Central Hospital, Qingdao University, Qingdao 266043, People’s Republic of China; 4Department of Clinical Laboratory, The People’s Hospital of Zhangqiu Area, Jinan 250200, People’s Republic of China; 5Department of Neurology, The People’s Hospital of Zhangqiu Area, Jinan 250200, People’s Republic of China; 6Department of Clinical Laboratory, Qingdao Municipal Hospital, Qingdao 266071, People’s Republic of ChinaCorrespondence: Huahui Li Email jigcgk24849@163.comObjective: Laryngeal cancer is a common malignant tumor in the ENT, of which laryngeal squamous cell carcinoma (LSCC) accounts for more than 90% of laryngeal cancer. The purpose of this study is to investigate the regulatory mechanism of lncRNA SNHG16 in LSCC.Materials and Methods: Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to measure mRNA expression. Cell Counting Kit (CCK-8), Transwell and luciferase reporter assays, flow cytometric analysis and Western blot analysis were used to investigate the function of lncRNA SNHG16 in LSCC.Results: SNHG16 expression was increased in LSCC tissues and cells. The abnormal expression of SNHG16 was associated with clinical stage and lymph node metastasis in LSCC patients. In addition, knockdown of SNHG16 restrained cell proliferation, migration and invasion in LSCC. More importantly, SNHG16 acted as a competitive endogenous RNA in LSCC and regulated FOXP4 expression by making miR-877-5p sponge. Further, SNHG16 promoted LSCC progression by interacting with miR-877-5p and FOXP4.Conclusion: LncRNA SNHG16 promotes the progression of LSCC by sponging miR-877-5p and upregulating FOXP4.Keywords: SNHG16, laryngeal squamous cell carcinoma, miR-877-5p, FOXP4

Published
2020

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