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The corrosion of metals poses a threat to the economy, the environment, and human health due to undesirable reactions and contaminated products. Corrosion inhibitors, including natural products, can play a key role in protecting metallic materials, especially under challenging conditions. In this study, the roots of the Inula viscosa plant were examined for their ability to act as corrosion inhibitors in a 1 M hydrochloric acid (HCl) solution. Different extracts of the plant were evaluated for their corrosion inhibition capacity in a 1 M HCl solution. The effectiveness of different plant extracts was assessed, including an aqueous extract, an ethanolic extract, and a combined water–ethanol extract. Compounds present in the roots of Inula viscosa were identified using high-performance liquid chromatography. The electrochemical properties of the extracts were studied using various techniques such as open circuit potential, electrochemical impedance spectroscopy, and potentiodynamic polarization. Additionally, surface analysis after immersion was performed using scanning electron microscopy. Electrochemical data revealed that Inula viscosa root (IVR) extracts acted as mixed-type corrosion inhibitors with pronounced cathodic characteristics. The inhibitory efficiency was closely related to the concentration of Inula viscosa (I. viscosa), showing a significant increase with higher concentrations. This resulted in a decrease in corrosion current and an increase in polarization resistance. Notably, inhibitory efficiency reached high levels, up to 97.7% in mixed extract which represents a mixture between water and ethanol. In our study, it was observed that the mixed extract (water + ethanol) allowed for a greater corrosion inhibition compared to the other solvents studied, 97.7%. Surface analyses confirmed the formation of an organic film layer on the steel surface, attributed to the bonding of functional groups and heteroatoms in I. viscosa components. Therefore, this study paves the way for the potential integration of I. viscosa as a promising corrosion inhibition material, offering durable protection against steel corrosion and opening avenues for various related applications.

Chenopodium ambrosioides aerial parts have been historically employed in traditional medicine for addressing various ailments such as headaches, abdominal discomfort, joint issues, and respiratory disorders, alongside treatments for lice and warts. This study aimed to conduct a comprehensive phytochemical analysis of C. ambrosioides and assess the acute and subacute toxicity of oral treatments using fractions in preclinical trials. Spectrophotometric analysis via LC–MS/MS was used to characterize the plant’s chemical composition. Acute toxicity evaluation followed Organisation for Economic Co-operation and Development code 42 guidelines, conducted on adult male and female Wistar strain mice. Subsequently, Swiss mice were divided into six groups for the subacute toxicity study, receiving oral doses of 200 mg/kg extracts and fractions for 28 days. Daily observations and biochemical analyses were performed, with LC–MS/MS revealing a diverse array of compounds including organic acids, flavonoids, phenolic acids, rutin, hesperidin, nicotiflorine, and fumaric acid. Results indicated no lethality or alterations in body weight in treated groups, though some organ weight changes were noted. Biochemical analyses demonstrated values within the normal range for all groups, suggesting that the treatments did not induce adverse effects. Acute and subacute treatments with fractions did not result in lethality or toxic alterations at therapeutic doses, implying the safety of the product at appropriate levels. This study underscores the potential of C. ambrosioides as a safe therapeutic option warranting further exploration.

Due to the environmental problems linked to the production of portland cement, the search for new more economic and non-polluting construction materials has become a current issue of interest. Geopolymers represent new types of “polymeric” materials of mineral nature. The aim of this work is to study the influence of the Na2SiO3/NaOH mass ratio and curing time on the mechanical and microstructural properties of fly-ash-based geopolymers GP-Fs. The samples were synthesized with different Na2SiO3/NaOH mass ratios (2.5, 4, and 6) and curing times (2, 12, and 24 h) at 60°C. The GP-F pastes elaborated were analyzed by scanning electron microscopy, Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD). The sample with the highest compressive strength and density and the lowest water absorption was produced with a Na2SiO3/NaOH mass ratio of 2.5 and a curing time of 24 h, which is GP1, as confirmed by FTIR and XRD analyses; in addition, it had a compact structure attributed to a higher pozzolanic reactivity. These optimized geopolymer pastes were studied to assess the durability test, evaluating the resistance to fire and acid attack. Fire resistance was assessed by heating the GP-F pastes to 800°C for 2 h, and acid resistance was studied by immersing specimens in a solution of 6% acetic acid (GP-F-CH3COOH) and 6% hydrochloric acid (GP-F-HCl) for 2 months. Physico-chemical and microstructural changes before and after heat and acid exposure were studied using several analyses. Samples exposed to an acidic environment display a completely porous shape with some micro-cracks, inducing a decrease of the residual compressive energy. FTIR analysis showed that the geopolymer gel deformed after heating to 800°C, and pores were formed in their structure due to evaporation of water.

Considering it contains a variety of physiologically active compounds, including flavonoids, common phenols, and essential oils (EOs), St. John’s wort (Hypericum perforatum L.) is a common plant in Bulgaria that is predominantly used in folk medicine to cure various disorders. Determining the chemical makeup of St. John’s wort inflorescences that were gathered from northern Bulgaria was the purpose of this investigation. The antioxidant activity of H. perforatum L. extracts was assessed using 1,1-diphenyl-2-picrilhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and Trolox equivalent antioxidant capacity (TEAC) tests on methanol extract. The amount of EO obtained by water distillation was 0.08%, with its main components (over 3%) being n-nonane (27.46%), β-sesquiphellandrene (11.17%), heptanal dimethyl acetal (5.94%), ethyl hexyl ketone (5.93%), undecane (3.75%), sabinene (3.3%), and tridecyl alcohol (3.1%). Methanol extracts were obtained from the inflorescences, with the total flavonoid content determined as 8.66 mg quercetin equivalents (QE)/mg and total phenolic content as 271.33 mg Gallic acid equivalent/g. The FRAP assay yielded 493.07 µmol/L of antioxidant activity, while the TEAC assay yielded 106.39 µmol/L, respectively. Our findings enable a comprehensive characterization of H. perforatum from Bulgaria and an assessment of its oil suitability for potential industrial applications. Additionally, the results could guide the selection of specimens for future targeted breeding efforts.

Artemisia herba-alba Asso is an endemic plant from North-East Morocco offering a diverse range of pharmacological, cosmetic, and agro-ecological uses. However, Artemisia herba-alba has not been well exploited in the field of plant protection (post-harvest diseases). This is why the main objective of the present study is to evaluate the antifungal and antioxidant activities of extracts or the essential oil of this plant. Antifungal activity was assessed against Botrytis cinerea, Penicillium expansum, and Penicillium digitatum using the agar dilution method for the aqueous extracts and fumigation for the essential oil. Antioxidant activity was assessed using the DPPH scavenging test and the FRAP ferric ion reduction capacity. The chemical composition of the extracts was determined by HPLC and that of the essential oil by GC-MS/MS. In addition, the polyphenol, flavonoid, and tannin content was determined using colorimetric methods. The results of this study showed that the total content of polyphenols (217.60 mg GAE/g E), flavonoids (43.59 mg QE/g E), and tannins (32.58 mg GAE/g E) was significantly higher (p ≤ 0.05) in the ethanolic extract than in the aqueous and hexanoic extracts. Moreover, the HPLC analysis of the aqueous extract revealed the presence of six compounds, namely, catechin, gallic acid, hydroxybenzoic acid, syringic acid, caffeic acid, and rutin. In addition, the ethanolic extract was found to contain seven phenolic compounds. On the one hand, 35 compounds were identified in the essential oil of Artemisia herba-alba, representing 99.7% of the total. According to this study, the dominant compounds in the essential oil of Artemisia herba-alba Asso are camphor (46.57%), endo-borneol (5.65%), eucalyptol (5.64%), and thymol (3.85%). Furthermore, the biological evaluation showed that the extracts and essential oil of Artemisia herba-alba have significant antioxidant and antibacterial activities. The findings show that Artemisia herba-alba is a plant that can be used as a source of antifungal chemicals to prevent putrefaction of foodstuffs and, more specifically, postharvest diseases.

Chenopodium ambrosioides, a member of the Chenopodiaceae family, is renowned for its toxic properties. Despite its toxicity, it has been traditionally utilized in various communities, particularly in pediatric contexts, for its vermifuge, antispasmodic, and antipyretic attributes. This study aims to unravel the phytochemical composition present in organic fractions and aqueous extracts obtained from the aerial components of C. ambrosioides. Furthermore, our objective is to evaluate the antioxidant activity of these extracts and fractions, coupled with a comprehensive examination of their toxicological effects. Polyphenols were quantified using the Folin–Ciocalteu reagent, flavonoids via the aluminum trichloride reagent AlCl3, and tannins using the vanillin method. Identification of bioactive compounds within the plant specimen was accomplished through GC-MS spectrophotometric analysis. The assessment of antioxidant activity employed DPPH, ferric (Fe3+) ion antioxidant reducing power (FRAP), ABTS, and TAC methods, with quercetin, catechin, and ascorbic acid serving as standards. Dermoprotective activity was studied using the ultraviolet absorption test. The GC-MS analysis conducted on the aqueous extracts (EAI and EAM) and assorted fractions (FCH, FE, FB, and FA) revealed the presence of diverse chemical families encompassing alcohols, acids, terpenes, steroids, and phenolic compounds. The components identified in the investigated samples, including trans-ascaridol glycol, palmitic acid, phenol, octadecadienoic acid, isoascaridol, eicosanoic acid, 2-methoxy-4-vinyl phenol, mexiletine, and thymol, are postulated as potential contributors to the observed antioxidant activity inherent in the plant extracts and fractions. Our findings highlight the remarkable antioxidant potential of Chenopodium ambrosioides, with the ethyl acetate fraction exhibiting the highest activity (IC50 = 0.54 mg/ml) in the DPPH test. In the FRAP and ABTS tests, the n-butanolic and ethyl acetate fractions demonstrated superior activity (IC50 = 4.43 mg/ml, 12.9 mg/ml and IC50 = 1.6 mg/ml, 4.54 mg/ml, respectively). Conversely, the TAC test revealed that the macerated aqueous extract displayed the highest activity (316.33 mg Eq AG/g), followed closely by the n-butanolic fraction (250.67 mg Eq AG/g). These outcomes can be attributed to the abundant presence of phenolic compounds in the n-butanolic and ethyl acetate fractions, as well as the macerated aqueous extract, playing a pivotal role in the observed antioxidant activity. Additionally, our investigation of the dermoprotective activity demonstrated robust efficacy in the ethyl acetate fraction (FE) and the n-butanolic fraction (FB) compared to the standard agents employed (ZnO and methyl salicylate). Overall, our comprehensive studies affirm that the extracts and fractions derived from C. ambrosioides manifest moderate antioxidant activities alongside significant dermoprotective potential, elucidated by the presence of phenolic compounds in moderate quantities within the plant.

Natural substances extracted from plants have been increasingly studied and recognized, recently. Essential oils (EOs), for example, possess antioxidant and antibacterial properties, enabling their application across different sectors like agro-food, pharmaceuticals, and cosmetics. In Morocco, exceptional plant diversity, mirroring the diversity of ecosystems, has not yet revealed all its secrets. Therefore, the aim of this study is to determine the chemical composition and evaluate the antibacterial and antioxidant activities of EOs from Thymus zygis L. ssp. gracilis and Eucalyptus camaldulensis Dehnh. collected in the El Hoceima and Mamora regions, respectively. The EOs were extracted by hydrodistillation employing a Clevenger-type apparatus. Gas chromatography/mass spectrometry (GC/MS) analyses identified 54 constituents representing 92.65% of the total for T. zygis and 55 components representing 99.60% for E. camaldulensis. The primary components found in the EO of T. zygis are δ-terpineol (27.64%), followed by δ-3-carene (15.7%), thymol (14.17%), and dehydrolinalool (4.99%). The main compounds in E. camaldulensis EO are 1,8-cineole (43.61%), γ-terpinene (11.71%), α-terpineol (10.58%), and p-cymene (4.93%). The antioxidant properties of these oils were investigated by utilization of the 2,2-diphenyl-1-picrylhydrazyl method and the ferric reducing antioxidant power (FRAP) test. The antibacterial activity was assessed against two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two Gram-negative bacteria (Micrococcus luteus and Escherichia coli). Both EOs showed significant antioxidant activity but were less effective than reference antioxidants quercetin and catechin. Antibacterial studies demonstrated strong activity of T. zygis and E. camaldulensis EOs against the studied bacteria, as well as good inhibitory properties (minimum inhibitory concentration).

The abundance of Panonychus citri McGregor 1916 (Acari: Tetranychidae) and its associated enemies (Euseius stipulatus Athias-Henriot, 1960; Typhlodromus sp.; Phytoseiulus persimilis Athias-Henriot, 1957) was studied on two 12-year-old citrus cultivars, specifically Clementine “Nules” (Citrus Clementina) and Valencia (Citrus sinensis), in the Gharb region of Morocco. Throughout the entire monitoring period in the Valencia late cultivar, the density of the spider mite P. citri on leaves was notably higher at 38.0% (n = 1,212 mobile forms). Predator P. persimilis exhibited a leaf occupancy of 25.0% (n = 812), followed by Typhlodromus sp. at 20.0% (n = 643). Conversely, the abundance of E. stipulatus was lower at 17.0% (n = 538). In the Nules variety, P. citri abundance recorded a higher percentage at 48.0% (n = 1,922). E. stipulatus emerged as the most abundant predator at 23.0% (n = 898), followed by P. persimilis with 16.0% (n = 639). Meanwhile, the population of Typlodromus sp. remained notably low at 13.0% (n = 498). Regarding the fluctuation of the different mites studied on the two cultivars across monitoring dates, the period from May 4 to June 1 was characterized by low temperatures and a diminished presence of mite populations (P. citri, E. stipulatus, Typhlodromus sp., and P. persimilis). However, from June 7 to June 19, characterized by high temperatures, a notable increase in the presence of mite populations was observed. As regards the effect of the variety on the different mites studied, the varietal impact was significant.

Inductively coupled plasma and microwave digestion are used in this article to analyze various food matrices. Different natural herbs are used in the recipes for the food items that the people of the Middle Eastern part of the world eat; they use these herbs as spices in different proportions. The study examines the potential effects on human health associated with consuming trace and toxic elements, such as Ni, Mn, Pb, Cd, Fe, Zn, Ca, Mg, K, and Na. The targeted spices include sumac, za’atar, black lemon, cumin seed, and kabsa powder; these were used in a variety of recipes for the food items the people of Saudi Arabia eat but not limited to fattier, kabsa, tabbouleh, humus, haysa Al tumreya, and other traditional foods. The herbs were collected from the Saudi Arabian market. For microwave digestion, Merck's ultra-pure nitric acid and 30% hydrogen peroxide were used. For elemental analysis, a highly sophisticated technique of inductively coupled plasma mass spectrometry was applied. The average potassium values in black lemon (Loomi), kabsa powder, cumin seed, za’atar, and sumac were 1,283, 1,248, 1,739, 500, and 500 mg/100 g, respectively. These spices help fulfill the human body’s daily requirements of elemental content.

Carvacrol is a major component of many essential oils of the genus Thymus, Satureja, and Origanum, determining their antimicrobial, antioxidant, and other properties. The aim of the present study was to investigate some physicochemical and biological properties of pure carvacrol. The surface tension and density were measured at six different temperatures (30, 40, 50, 60, 70, and 80°C). The surface tension values were between 53.11 and 60.38 mN/m, while density varied from 0.978 to 0.99 kg/m3. The antibacterial activity against seven pathogenic and conditionally pathogenic bacteria was investigated. The diameter of the inhibition zones was determined in the range of 3.9–4.9 mm. The antioxidant activity was determined by the oxygen radical absorbance capacity (1687.0 µmol TE/mL) method.

Morus nigra, black mulberry, is the most attractive among mulberry species. The study aimed to determine human health-promoting content and antifungal activity in fruits of seven black mulberry genotypes grown in the Tortum district of Turkey. The genotypes exhibited significant differences in most of the human health-promoting content. Among individual sugars and organic acids, glucose (6.98–8.03 g/100 g fresh weight base) and malic acid (6.33–10.45 g/100 g fresh weight base) were predominant. The ascorbic acid content of genotypes was found between 18.13 and 26.77 mg/100 g fresh weight base, indicating that black mulberry fruits had moderate ascorbic acid content. Total phenolic content, total antioxidant capacity, and total anthocyanin contents in fruit extracts of seven black mulberry genotypes were also investigated. The results showed that the levels of the aforementioned parameters changed depending on genotypes. The total phenolic and total anthocyanin contents were in the range of 1,656–2,348 μg gallic acid equivalent (GAE)/g and 623–804 μg C3GE (cyanidin-3-glucoside equivalent)/g, respectively. The genotypes had antioxidant capacity between 17.41 and 3.86 μg/mL and between 10.08 and 14.11 μmol TE/g measured by the 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assay, respectively. Some of the genotypes (TOR-1 and TOR-2) had high antifungal activity particularly against C. albicans. It was determined that the people living in the region traditionally use mulberries as blood enhancer, immune system booster, and mouth lesion treatments to protect themselves against different types of cancers and against inflammation. The present results confirm that attractive fruits of black mulberry are a rich natural source of phenolic antioxidants and can contribute to the dietary intake of antioxidants, depending on genotypes.

Berberis hispanica Boiss. & Reut (B. hispanica) belongs to the family Berberidaceae, which is currently used in traditional medicines. This article aimed to study the phytochemical composition and acute and subacute toxicity of B. hispanica extract in rats. The phytochemical composition of B. hispanica extract was characterized using GC-MS. The acute toxicity was investigated in vivo via the oral administration of single doses including 400, 800, 1,000, 1,200, and 1,400 mg/kg for 14 days. The subchronic toxicity was studied through oral administration of 400 mg/kg for 60 days. The findings of the phytochemical analysis of B. hispanica extract showed the presence of various phytochemical compounds. Acute toxicity results revealed serious clinical symptoms and mortalities in rats treated with 800 mg/kg up to a maximum of 1,400 mg/kg. With acute toxicity, subchronic toxicity results showed also serious signs of toxicity including biochemical and histological alterations in animals treated with 1,400 mg/kg. B. hispanica extract revealed to be toxic in rats orally treated under both subacute (>400 mg/kg) and subchronic toxicity conditions (400 mg/kg). The outcome of this study serves the society as it provides toxicological evidence of B. hispanica used in alternative medicines

Autoimmune diseases are pathological conditions in which the immune system mistakenly attacks its own tissues. This study evaluates the performance of two techniques, which are identifiers of autoantibody specifics: immunoblot and immunodot. This study was conducted in 300 patients of whom 62 were tested positive for antinuclear antibodies. The patients were initially screened for antinuclear antibodies using indirect immunofluorescence. Then, the identification of specific autoantibodies such as anti-extractable nuclear antigens (ENAs) was carried out using the immunoblot and immunodot techniques. The results showed that immunoblot and immunodot did not present a significant difference in their sensitivity against anti-SSA/52, SSB, CENP-B, PCNA, U1-snRNP, Jo-1, Pm-scl, and Mi-2 (p > 0.05). However, the two techniques showed a significant difference in their sensitivity toward autoantibodies anti-DNAn, anti-histone, anti-SmD1, and anti-ds-DNA (p < 0.05). The immunoblot data were in complete accordance with the immunodot data (100%) regarding the detection of autoantibodies such as anti SSA/52, SSB, CENP-B, PCNA, U1-snRP, Jo-1, Pm-scl, and Mi-2, 80% regarding SmD1, and 75% concerning ds-DNA. We should certainly pay closer attention to the efficiency of the techniques used in the diagnosis of autoimmune diseases.

Orotic acid esters and several N-arylhydrazones derivatives were synthesized and evaluated for their stem cell proliferation potential with human stromal stem cells (hMSCs) which was never studied before. Some of the derivatives exhibit convincing upregulation of proliferation which is believed to be due to substitution on the aromatic ring.

To investigate the phytochemical composition, acute and sub-acute toxicity of the aqueous extract of B. dioica roots. The phytochemical analysis was performed using gas chromatography-mass spectrometry (GC-MS). The acute toxicity of the aqueous extract of B.dioica roots was assessed in mice with single doses ranging from 250 to 1000 mg/kg for 14 days. The sub-acute toxicity was carried out with repeated doses ranging from 64.5 to 250 mg/kg for 28 days. Histopathological changes and markers of renal and liver function were investigated. The results of GC-MS analysis showed the presence of interesting phytoconstituents. The clinical symptoms and mortalities that occurred in treated mice were more remarkable due to the increasing sample concentration of the studied extract. However, no mortalities, or histopathological, or biochemical disturbances were observed even at the maximal dose administered (250 mg/kg). The outcome of the present work suggests that the treatment of animals with single doses of B. dioica roots extract higher than 250 mg/kg produces significant toxicities, however, treatment with repeated doses up to 250 mg/kg for 28 days seems to be safe for animals.

C16H26N2S, monoclinic, P21/n (no. 14), a = 6.4855(4) Å, b = 20.1110(12) Å, c = 11.2575(7) Å, β = 92.014(2)°, V = 1467.41(16) Å3, Z = 4, Rgt(F) = 0.0324, wRref(F2) = 0.0889, T = 200 K.

C19H30N2O2S, monoclinic, P21/n (no. 14), a = 6.4977(5) Å, b = 28.728(2) Å, c = 10.1806(8) Å, β = 101.549(3)°, V = 1861.9(2) Å3, Z = 4, Rgt(F) = 0.0460, wRref(F2) = 0.1213, T = 296 K.

C8H18CuNa2O11, monoclinic, P21/n (no. 14), a = 9.9625(5) Å, b = 14.0145(7) Å, c = 11.3376(6) Å, β = 97.602(2)°, V = 1569.04(14) Å3, Z = 4, Rgt(F) = 0.0178, wRref(F2) = 0.0482, T = 200 K.

Herein we have determined the heavy metals concentrations (Pb, Cd, and Ni) estimated in River Swat water, irregated soils samples, fodder plant's parts, farmed animal milk/meat samples and human blood from two location Mingora and Barikot in District Swat, Pakistan, using atomic absorption spectrophotometer. Heavy metals such as Cd, Pb, and Ni were found 0.016 ± 0.008, 0.50 ± 0.03, and 0.022 ± 0.004 mg/L in Mingora drain water, respectively, while in the Barikot sample the aforementioned metal were in the range of 0.014 ± 0.01, 0.29 ± 0.02, and 0.108 ± 0.08 mg/L. In the field water samples of Mingora the Cd, Pb and Ni were detected in the range of 0.008 ± 0.001, 0.65 ± 0.05 and 0.032 ± 0.006 mg/L, respectively, while in the field water samples of Barikot contained Cd = 0.016 ± 0.007 mg/L, Pb = 0.48 ± 0.04 mg/L and Ni = 0.043 ± 0.003 mg/L. Drain soil of Mingora contained the highest concentrations of heavy metals, with Cd = 3.9 ± 0.62 mg/kg, Pb = 45.5 ± 3.2 mg/kg, and Ni = 19.95 ± 2.1 mg/kg, whereas field soil contained Cd = 1.6 ± 0.44 mg/kg, Pb = 54.5 ± 2.26 mg/kg, and Ni = 34.75 ± 3.2 mg/kg. Cd in the Barikot drain soil was 3.95 ± 0.71 mg/kg, while it was 0.8 ± 0.06 mg/kg in the field soil. Lead in the same drain sample was 33 ± 3.23 mg/kg, while it was 72 ± 4.15 mg/kg in the field sample. Ni concentrations in Barikot drain soil samples was 13.85 ± 1.1 mg/kg, while 6.9 ± 0.8 mg/kg was detected in field samples. From the results it can be concluded that appreciable concentrations of heavy metals are present in milk and meat samples of farmed animals whereas significant quantities are also there in human blood samples indicating the transport of the selected metals from water through fodder plants into human. However, it may not be the sole reason heavy metal contaminations in human blood other factors like inhalation etc. may also be equally responsible. [ABSTRACT FROM AUTHOR]

C9H11ClN4O2, orthorhombic, Pbca (no. 61), a = 8.9735(4) Å, b = 12.2946(7) Å, c = 19.3751(11) Å, V = 2137.6(2) Å3, Z = 8, Rgt(F) = 0.0355, wRref(F2) = 0.1087, T = 200(2) K.

C14H11N3S2, triclinic, P1̅ (no. 2), a = 5.7607(5)Å, b = 9.8397(9) Å, c = 12.0920(11) Å, α = 72.899(4)°, β = 81.833(4)°, γ = 86.796(5)°, V = 648.40(10) Å3, Z = 2, Rgt(F) = 0.0295, wRref(F2) = 0.0797, T = 200(2) K.

C8H13N3S2, triclinic, P1̅ (no. 2), a = 7.4803(3) Å, b = 8.4562(3) Å, c = 9.3531(3) Å, α = 67.688(1)°, β = 74.017(1)°, γ = 85.008(1)°, V = 526.07(3) Å3, Z = 2, Rgt(F) = 0.0256, wRref(F2) = 0.0736, T = 200 K.

Berberis hispanica Boiss. & Reut (B. hispanica) belongs to the family Berberidaceae, which is currently used in traditional medicines. This article aimed to study the phytochemical composition and acute and subacute toxicity of B. hispanica extract in rats. The phytochemical composition of B. hispanica extract was characterized using GC-MS. The acute toxicity was investigated in vivo via the oral administration of single doses including 400, 800, 1,000, 1,200, and 1,400 mg/kg for 14 days. The subchronic toxicity was studied through oral administration of 400 mg/kg for 60 days. The findings of the phytochemical analysis of B. hispanica extract showed the presence of various phytochemical compounds. Acute toxicity results revealed serious clinical symptoms and mortalities in rats treated with 800 mg/kg up to a maximum of 1,400 mg/kg. With acute toxicity, subchronic toxicity results showed also serious signs of toxicity including biochemical and histological alterations in animals treated with 1,400 mg/kg. B. hispanica extract revealed to be toxic in rats orally treated under both subacute (>400 mg/kg) and subchronic toxicity conditions (400 mg/kg). The outcome of this study serves the society as it provides toxicological evidence of B. hispanica used in alternative medicines. [ABSTRACT FROM AUTHOR]

C8H18CuNa2O11, monoclinic, P21/n (no. 14), a=9.9625(5) Å, b=14.0145(7) Å, c=11.3376(6) Å, β=97.602(2)°, V =1569.04(14) Å3, Z =4, Rgt(F)=0.0178, wRref(F2)=0.0482, T =200 K. [ABSTRACT FROM AUTHOR]

C16H26N2S, monoclinic, P21/n (no. 14), a = 6.4855(4) Å, b = 20.1110(12) Å, c = 11.2575(7) Å, β = 92.014(2)°, V = 1467.41(16) Å3, Z = 4, Rgt(F) = 0.0324, wRref(F2) = 0.0889, T = 200 K. [ABSTRACT FROM AUTHOR]

C19H30N2O2S, monoclinic, P21/n (no. 14), a = 6.4977(5) Å, b = 28.728(2) Å, c = 10.1806(8) Å, β = 101.549(3)°, V = 1861.9(2) Å3, Z = 4, Rgt(F) = 0.0460, wRref(F2) = 0.1213, T = 296 K. [ABSTRACT FROM AUTHOR]

C9H11ClN4O2, orthorhombic, Pbca (no. 61), a = 8.9735(4) Å, b = 12.2946(7) Å, c = 19.3751(11) Å, V = 2137.6(2) Å3, Z = 8, Rgt(F) = 0.0355, wRref(F2) = 0.1087, T = 200(2) K. [ABSTRACT FROM AUTHOR]

C14H11N3S2, triclinic, P1̅ (no. 2), a = 5.7607(5)Å, b = 9.8397(9) Å, c = 12.0920(11) Å, α = 72.899(4)°, β = 81.833(4)°, γ = 86.796(5)°, V = 648.40(10) Å3, Z = 2, Rgt( F) = 0.0295, wRref( F2) = 0.0797, T = 200(2) K. [ABSTRACT FROM AUTHOR]

C8H13N3S2, triclinic, P1̅ (no. 2), a = 7.4803(3) Å, b = 8.4562(3) Å, c = 9.3531(3) Å, α = 67.688(1)°, β = 74.017(1)°, γ = 85.008(1)°, V = 526.07(3) Å3, Z = 2, Rgt (F) = 0.0256, wRref (F2 ) = 0.0736, T = 200 K. [ABSTRACT FROM AUTHOR]

C9H11ClN4O2, orthorhombic, Pbca (no. 61), a = 8.9735(4) Å, b = 12.2946(7) Å, c = 19.3751(11) Å, V = 2137.6(2) Å3, Z = 8, Rgt(F) = 0.0355, wRref(F2) = 0.1087, T = 200(2) K. [ABSTRACT FROM AUTHOR]

C14H11N3S2, triclinic, P1̅ (no. 2), a = 5.7607(5)Å, b = 9.8397(9) Å, c = 12.0920(11) Å, α = 72.899(4)°, β = 81.833(4)°, γ = 86.796(5)°, V = 648.40(10) Å3, Z = 2, Rgt( F) = 0.0295, wRref( F2) = 0.0797, T = 200(2) K. [ABSTRACT FROM AUTHOR]

C8H18CuNa2O11, monoclinic, P21/n (no. 14), a=9.9625(5) Å, b=14.0145(7) Å, c=11.3376(6) Å, β=97.602(2)°, V =1569.04(14) Å3, Z =4, Rgt(F)=0.0178, wRref(F2)=0.0482, T =200 K. [ABSTRACT FROM AUTHOR]

C8H13N3S2, triclinic, P1̅ (no. 2), a = 7.4803(3) Å, b = 8.4562(3) Å, c = 9.3531(3) Å, α = 67.688(1)°, β = 74.017(1)°, γ = 85.008(1)°, V = 526.07(3) Å3, Z = 2, Rgt (F) = 0.0256, wRref (F2 ) = 0.0736, T = 200 K. [ABSTRACT FROM AUTHOR]