1. EpCAM-independent capture of circulating tumor cells with a 'universal CTC-chip'.
- Author
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Chikaishi Y, Yoneda K, Ohnaga T, and Tanaka F
- Subjects
- Antigens, Neoplasm metabolism, Cell Line, Tumor, Epithelial Cell Adhesion Molecule metabolism, Flow Cytometry instrumentation, Flow Cytometry methods, Humans, Membrane Glycoproteins metabolism, Microfluidic Analytical Techniques instrumentation, Microfluidic Analytical Techniques methods, Cell Separation instrumentation, Cell Separation methods, Lab-On-A-Chip Devices, Neoplastic Cells, Circulating metabolism, Neoplastic Cells, Circulating pathology
- Abstract
Capture of circulating tumor cells (CTCs), which are shed from the primary tumor site and circulate in the blood, remains a technical challenge. CellSearch® is the only clinically approved CTC detection system, but has provided only modest sensitivity in detecting CTCs mainly because epithelial cell adhesion molecule (EpCAM)-negative tumor cells may not be captured. To achieve more sensitive CTC‑capture, we have developed a novel microfluidic platform, a 'CTC-chip' comprised of light-curable resins that has a unique advantage in that any capture antibody is easily conjugated. In the present study, we showed that EpCAM-negative tumor cells as well as EpCAM-positive cells were captured with the novel 'universal CTC-chip' as follows: i) human lung cancer cells (PC-9), with strong EpCAM expression, were efficiently captured with the CTC-chip coated with an anti-EpCAM antibody (with an average capture efficiency of 101% when tumor cells were spiked in phosphate‑buffered saline (PBS) and 88% when spiked in blood); ii) human mesothelioma cells (ACC-MESO-4), with no EpCAM expression but with podoplanin expression, were captured with the CTC-chip coated with an anti-podoplanin antibody (average capture efficiency of 78% when tumor cells were spiked in PBS and 38% when spiked in blood), whereas ACC-MESO-4 cells were not captured with the CTC-chip coated with the anti-EpCAM antibody. These results indicate that the novel 'CTC-chip' can be useful in sensitive EpCAM-independent detection of CTCs, which may provide new insights into personalized medicine.
- Published
- 2017
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