1. Anti-leukaemic effects of two extract types of Lactuca sativa correlate with the activation of Chk2, induction of p21, downregulation of cyclin D1 and acetylation of alpha-tubulin.
- Author
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Gridling M, Popescu R, Kopp B, Wagner KH, Krenn L, and Krupitza G
- Subjects
- Acetylation, Blotting, Western, Cell Cycle Proteins metabolism, Cell Line, Tumor, Checkpoint Kinase 2, Cyclin D1 drug effects, Cyclin D1 metabolism, Cyclin-Dependent Kinase Inhibitor p21 drug effects, Cyclin-Dependent Kinase Inhibitor p21 metabolism, Down-Regulation, Humans, Protein Serine-Threonine Kinases drug effects, Protein Serine-Threonine Kinases metabolism, Proto-Oncogene Mas, Tubulin drug effects, Tubulin metabolism, Antineoplastic Agents, Phytogenic pharmacology, Cell Cycle Proteins drug effects, Cell Proliferation drug effects, Lactuca chemistry, Plant Extracts pharmacology
- Abstract
The water extract of the lettuce Lactuca sativa, but not the ethyl acetate extract, inhibited the growth of HL-60 leukaemia cells and MCF-7 breast cancer cells. This correlated with the activation of checkpoint kinase 2 (Chk2), the induction of the tumour suppressor p21, and the severe downregulation of the proto-oncogene cyclin D1. The ethyl acetate extract, but not the water extract, induced HL-60 cell death, which correlated with the acetylation of alpha-tubulin. The acetylation of alpha-tubulin is indicative for microtubuli stabilisation such as induced by taxol. The calculated amount for human intake would require approximately 3 kg lettuce to reach the required concentration shown to inhibit 50% HL-60 proliferation.
- Published
- 2010
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