1. Propranolol treatment of infantile hemangioma endothelial cells: A molecular analysis
- Author
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Mary Lacaze, Laura E. Boucheron, Robert Pham, Brad A. Bryan, Victor Kokta, Clarissa N. Amaya, Rebecca K. Rowntree, Arlynn F. Mulne, Dianne C. Mitchell, Jessica M. Stiles, and Joyce Bischoff
- Subjects
Cancer Research ,medicine.medical_specialty ,Angiogenesis ,infantile hemangioma ,Propranolol ,Hemangioma ,03 medical and health sciences ,chemistry.chemical_compound ,angiogenesis ,0302 clinical medicine ,Immunology and Microbiology (miscellaneous) ,Internal medicine ,medicine ,propranolol ,030304 developmental biology ,0303 health sciences ,business.industry ,General Medicine ,Articles ,Cell cycle ,medicine.disease ,endothelial cells ,3. Good health ,Vascular endothelial growth factor B ,Vascular endothelial growth factor ,Endothelial stem cell ,Vascular endothelial growth factor A ,Endocrinology ,chemistry ,030220 oncology & carcinogenesis ,business ,medicine.drug - Abstract
Infantile hemangiomas (IHs) are non-malignant, largely cutaneous vascular tumors affecting approximately 5-10% of children to varying degrees. During the first year of life, these tumors are strongly proliferative, reaching an average size ranging from 2 to 20 cm. These lesions subse- quently stabilize, undergo a spontaneous slow involution and are fully regressed by 5 to 10 years of age. Systemic treatment of infants with the non-selective β-adrenergic receptor blocker, propranolol, has demonstrated remarkable efficacy in reducing the size and appearance of IHs. However, the mechanism by which this occurs is largely unknown. In this study, we sought to understand the molecular mechanisms underlying the effectiveness of β blocker treatment in IHs. Our data reveal that propranolol treatment of IH endothelial cells, as well as a panel of normal primary endothelial cells, blocks endothelial cell proliferation, migration, and formation of the actin cyto- skeleton coincident with alterations in vascular endothelial growth factor receptor-2 (VEGFR-2), p38 and cofilin signaling. Moreover, propranolol induces major alterations in the protein levels of key cyclins and cyclin-dependent kinase inhibitors, and modulates global gene expression patterns with a particular affect on genes involved in lipid/sterol metabolism, cell cycle regulation, angiogenesis and ubiquitination. Interestingly, the effects of propranolol were endothelial cell-type independent, affecting the properties of IH endothelial cells at similar levels to that observed in neonatal dermal microvascular and coronary artery endothelial cells. This data suggests that while propranolol markedly inhibits hemangioma and normal endothelial cell function, its lack of endothelial cell specificity hints that the efficacy of this drug in the treatment of IHs may be more complex than simply blockage of endothelial function as previously believed.
- Published
- 2012