1. An epiblast stem cell-derived multipotent progenitor population for axial extension.
- Author
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Edri, Shlomit, Hayward, Penny, Baillie-Johnson, Peter, Steventon, Benjamin J., and Arias, Alfonso Martinez
- Subjects
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MESODERM , *EMBRYONIC stem cells , *MAMMALIAN embryos , *STEM cells , *EPIBLAST , *SPINAL cord - Abstract
The caudal lateral epiblast of mammalian embryos harbours bipotent progenitors that contribute to the spinal cord and the paraxial mesoderm in concert with the body axis elongation. These progenitors, called neural mesodermal progenitors (NMPs), are identified as cells that co-express Sox2 and T/brachyury, a criterion used to derive NMP-like cells from embryonic stem cells in vitro. However, unlike embryonic NMPs, these progenitors do not selfrenew. Here, we find that the protocols that yield NMP-like cells in vitro initially produce a multipotent population that, in addition to NMPs, generates progenitors for the lateral plate and intermediate mesoderm. We show that epiblast stem cells (EpiSCs) are an effective source of these multipotent progenitors, which are further differentiated by a balance between BMP and Nodal signalling. Importantly, we show that NMP-like cells derived from EpiSCs exhibit limited self-renewal in vitro and a gene expression signature like their embryonic counterparts. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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