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Start Over Author "Shen, Arthur" Publisher cold spring harbor laboratory press
2 results on '"Shen, Arthur"'

1. Comparative genome analysis of Vibrio vulnificus, a marine pathogen

Author

Chung-Yung Chen, Keh-Ming Wu, Yo-Cheng Chang, Chuan-Hsiung Chang, Hui-Chi Tsai, Tsai-Lien Liao, Yen-Ming Liu, Hsiang-Ju Chen, Shen, Arthur Bo-Ting, Jian-Chiuan Li, Teh-Li Su, Chung-Ping Shao, Chung-Te Lee, Lien-I Hor, and Shih-Feng Tsai

Subjects
Evolution -- Genetic aspects, Genetic research -- Comparative analysis, Genomes -- Comparative analysis, Genomes -- Research, Infection -- Causes of, Mortality -- Causes of, Pathogenic microorganisms -- Genetic aspects, Vibrio -- Genetic aspects, Mortality -- Taiwan, Health
Abstract

Research has been conducted on etiologic agent of human mortality from seafood-borne infections, Vibrio vulnificus. The authors have investigated the evolution of this pathogen via the use of comparative analysis and whole-genome sequencing.

Published
2003

2. Comparative genome analysis of Vibrio vulnificus, a marine pathogen.

Author

Chen CY, Wu KM, Chang YC, Chang CH, Tsai HC, Liao TL, Liu YM, Chen HJ, Shen AB, Li JC, Su TL, Shao CP, Lee CT, Hor LI, and Tsai SF

Subjects
Amino Acid Sequence genetics, Base Sequence, Chromosomes, Bacterial genetics, Conjugation, Genetic genetics, Evolution, Molecular, Integrons genetics, Molecular Sequence Data, Multigene Family genetics, Plasmids genetics, Sequence Analysis, DNA methods, Vibrio cholerae genetics, Vibrio cholerae pathogenicity, Virulence Factors genetics, Genome, Bacterial, Vibrio vulnificus genetics, Vibrio vulnificus pathogenicity
Abstract

The halophile Vibrio vulnificus is an etiologic agent of human mortality from seafood-borne infections. We applied whole-genome sequencing and comparative analysis to investigate the evolution of this pathogen. The genome of biotype 1 strain, V. vulnificus YJ016, was sequenced and includes two chromosomes of estimated 3377 kbp and 1857 kbp in size, and a plasmid of 48,508 bp. A super-integron (SI) was identified, and the SI region spans 139 kbp and contains 188 gene cassettes. In contrast to non-SI sequences, the captured gene cassettes are unique for any given Vibrio species and are highly variable among V. vulnificus strains. Multiple rearrangements were found when comparing the 5.3-Mbp V. vulnificus YJ016 genome and the 4.0-Mbp V. cholerae El Tor N16961 genome. The organization of gene clusters of capsular polysaccharide, iron metabolism, and RTX toxin showed distinct genetic features of V. vulnificus and V. cholerae. The content of the V. vulnificus genome contained gene duplications and evidence of horizontal transfer, allowing for genetic diversity and function in the marine environment. The genomic information obtained in this study can be applied to monitoring vibrio infections and identifying virulence genes in V. vulnificus.

Published
2003
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