1. Staphylococcus aureus RNAIII coordinately represses the synthesis of virulence factors and the transcription regulator Rot by an antisense mechanism
- Author
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Nadia Bendridi, Pierre Fechter, Maria Possedko, François Vandenesch, Eric Huntzinger, Sandrine Boisset, Anne Catherine Helfer, Pascale Romby, Yvonne Benito, Thomas Geissmann, Clément Chevalier, Alain Jacquier, Christine Gaspin, Immunité infection vaccination (I2V), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR128-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Architecture et réactivité de l'ARN (ARN), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Génétique des Interactions Macromoléculaires, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Unité de Biométrie et Intelligence Artificielle, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Unité de gestion du département de biométrie et intelligence artificielle, and Institut National de la Recherche Agronomique (INRA)
- Subjects
Transcription, Genetic ,RNAIII ,RNase III ,MESH: Base Sequence ,MESH: Quorum Sensing ,Staphylococcus aureus] ,MESH: Staphylococcus aureus ,MESH: RNA, Antisense ,MESH: Bacterial Proteins ,3' Untranslated Regions ,Genetics ,0303 health sciences ,MESH: Gene Expression Regulation, Bacterial ,Effector ,Hydrolysis ,Quorum Sensing ,MESH: 3' Untranslated Regions ,Antisense RNA ,antisense regulation ,RNA, Bacterial ,MESH: Nucleic Acid Conformation ,MESH: RNA, Bacterial ,translational repression ,MESH: Hydrolysis ,Research Paper ,Staphylococcus aureus ,Virulence Factors ,Molecular Sequence Data ,Virulence ,Biology ,MESH: Sequence Homology, Nucleic Acid ,03 medical and health sciences ,Eukaryotic translation ,Bacterial Proteins ,Sequence Homology, Nucleic Acid ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,RNA, Antisense ,RNA, Messenger ,Psychological repression ,Gene ,030304 developmental biology ,MESH: RNA, Messenger ,MESH: Virulence Factors ,MESH: Molecular Sequence Data ,Base Sequence ,030306 microbiology ,MESH: Transcription, Genetic ,RNA ,Gene Expression Regulation, Bacterial ,virulence ,Regulatory RNA ,Nucleic Acid Conformation ,Developmental Biology - Abstract
RNAIII is the intracellular effector of the quorum-sensing system in Staphylococcus aureus. It is one of the largest regulatory RNAs (514 nucleotides long) that are known to control the expression of a large number of virulence genes. Here, we show that the 3′ domain of RNAIII coordinately represses at the post-transcriptional level, the expression of mRNAs that encode a class of virulence factors that act early in the infection process. We demonstrate that the 3′ domain acts primarily as an antisense RNA and rapidly anneals to these mRNAs, forming long RNA duplexes. The interaction between RNAIII and the mRNAs results in repression of translation initiation and triggers endoribonuclease III hydrolysis. These processes are followed by rapid depletion of the mRNA pool. In addition, we show that RNAIII and its 3′ domain mediate translational repression of rot mRNA through a limited number of base pairings involving two loop–loop interactions. Since Rot is a transcriptional regulatory protein, we proposed that RNAIII indirectly acts on many downstream genes, resulting in the activation of the synthesis of several exoproteins. These data emphasize the multitude of regulatory steps affected by RNAIII and its 3′ domain in establishing a network of S. aureus virulence factors.
- Published
- 2007