1. Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis
- Author
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Virginia Andrade, Jian Bai, Neetu Gupta-Rossi, Ana Joaquina Jimenez, Cédric Delevoye, Christophe Lamaze, Arnaud Echard, Trafic membranaire et Division cellulaire - Membrane Traffic and Cell Division, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Sorbonne Université (SU), Dynamique de l’organisation intra-cellulaire, Biologie Cellulaire et Cancer, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), BioImaging Cell and Tissue Core Facility (PICT-IBiSA), Institut Curie [Paris], Structure et compartimentation membranaire, Compartimentation et dynamique cellulaires (CDC), Chimie biologique des membranes et ciblage thérapeutique (CBMCT - UMR 3666 / U1143), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), This work was supported by Institut Pasteur (A.E.), CNRS (A.E.), ANR Cytosign, ANR SeptScort (A.E.), ANR MOTICAV (C.L.), ANR CAV-SM (C.L.), Fondation pour le Recherche Médicale: Recherche soutenue par la FRM EQU202103012627 (A.E.), French National Research Agency through the 'Investments for the Future' program, France-BioImaging, ANR-10-INSB-04 (C.D.), Cell and Tissue Imaging core facility (PICT-IBiSA), member of the France-BioImaging national research infrastructure, supported by the Labex Cell(n)Scale (ANR-10-LBX-0038) part of the IDEX PSL (ANR-10-IDEX-0001-02 PSL) (C.D. and C.L.), Doctoral School Complexité du Vivant ED515, contrat n° 2828/2017 (V.A.), La Ligue Contre le Cancer (4ème année de thèse) (V.A.), Pasteur-Paris University (PPU) international PhD program (J.B.), Fondation ARC pour la recherche sur le cancer (DOC20180507410) (J.B.), Programme Labellisé PGA1-RF20170205456 (C.L.), and CEFIPRA, project 6301-1., ANR-16-CE13-0004,CYTOSIGN,Polarité épithéliale et signalisation au cours de la cytodiérèse des cellules animales(2016), ANR-20-CE11-0014,SeptScort,Interaction spatiale et fonctionnelle entre septines et Escrt pendant la cytocinèse: une approche multi-échelle(2020), ANR-17-CE13-0020,MOTICAV,Cavéoles et Tension Membranaire dans la Migration Cellulaire(2017), ANR-20-CE13-0002,CAV-SM,Un rôle nouveau de la cavéoline dans le transport de la sphingomyéline à la membrane plasmique(2020), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), Collège Doctoral, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Agence Nationale de la Recherche: CytosignAgence Nationale de la Recherche: SeptScortAgence Nationale de la Recherche: ANR-10-INSB-04Agence Nationale de la Recherche: ANR-10-LBX-0038Agence Nationale de la Recherche: ANR-10-IDEX-0001-02 PSLInstitut PasteurFondation ARC pour la Recherche sur le Cancer: DOC20180507410La Ligue Contre Le CancerDoctoral School Complexité du Vivant ED515: 2828/2017CNRSPasteur-Paris University (PPU) international PhD program
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Multidisciplinary ,[SDV]Life Sciences [q-bio] ,macromolecular substances - Abstract
During cytokinesis, the intercellular bridge (ICB) connecting the daughter cells experiences pulling forces, which delay abscission by preventing the assembly of the ESCRT scission machinery. Abscission is thus triggered by tension release, but how ICB tension is controlled is unknown. Here, we report that caveolae, which are known to control membrane tension upon mechanical stress in interphase cells, are located at the midbody, at the abscission site and at the ICB/cell interface in dividing cells. Functionally, the loss of caveolae delays ESCRT-III recruitment during cytokinesis and impairs abscission. This is the consequence of a 2-fold increase of ICB tension measured by laser ablation, associated with a local increase in myosin II activity at the ICB/cell interface. We thus propose that caveolae buffer membrane tension and limit contractibility at the ICB to promote ESCRT-III assembly and cytokinetic abscission. Altogether, this work reveals an unexpected connection between caveolae and the ESCRT machinery and the first role of caveolae in cell division.TEASERCaveolae limit the tension in the intercellular bridge during cytokinesis to enable ESCRT-III assembly and successful abscission.
- Published
- 2022
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