1. A methyl-seq tool to capture genomic imprinted loci
- Author
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Hubert Jean-Noël, Iannuccelli Nathalie, Cabau Cédric, Jacomet Eva, Billon Yvon, Serre Rémy-Felix, Vandecasteele Céline, Donnadieu Cécile, and Demars Julie
- Abstract
Genomic imprinting represents an original model of epigenetic regulation resulting in a parent-of-origin expression. Despite the critical role of imprinted genes in mammalian growth, metabolism and neuronal function, there is no molecular tool specifically targeting them for a systematic evaluation. Here, we optimized and compared to bisulfite-based standard a novel methyl-seq system to capture 165 candidate regions for genomic imprinting and ultimately detect parent-of-origin methylation, the main hallmark of imprinting.
- Published
- 2023