1. Gene Expression Profiles of the Aurora Family Kinases
- Author
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Chang Tze Ricky Yu, Li Jen Su, Shih-Lan Hsu, Jin Mei Lai, Wey Jinq Lin, Fen Hwa Wong, Yow-Ling Shiue, Jiunn Chyi Wu, Su Liang Chen, Chi Ying F. Huang, Yong Shiang Lin, Hsu Hua Yeh, and Hsiao Sheng Liu
- Subjects
Esophageal Neoplasms ,Aurora B kinase ,Aurora inhibitor ,macromolecular substances ,Biology ,Protein Serine-Threonine Kinases ,Article ,Aurora Kinases ,Genetics ,Tumor Cells, Cultured ,Humans ,Aurora Kinase C ,RNA, Messenger ,RNA, Neoplasm ,Molecular Biology ,Mitosis ,Regulation of gene expression ,Kinase ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Profiling ,Cell cycle ,Cell biology ,Gene expression profiling ,Gene Expression Regulation, Neoplastic ,enzymes and coenzymes (carbohydrates) ,embryonic structures ,biological phenomena, cell phenomena, and immunity ,HeLa Cells - Abstract
The evolutionarily conserved Aurora family kinases, a family of mitotic serine/threonine kinases, has three members in humans (Aurora-A, -B and -C). Overexpression of Aurora family members, particularly Aurora-A, has been reported in many human cancers and cell lines. In this study, we present evidence based on comparative gene expression analysis via quantitative RT-PCR to delineate the relative contributions of these kinases in 60 cell lines and statistical analysis in five different human cancer microarray datasets. The analysis demonstrated the selective upregulation of these Aurora members in various cancers. In general, Aurora-A exhibited the highest expression levels, with substantially decreased quantities of the Aurora-C transcript detected relative to Aurora-A and -B. Moreover, to characterize the roles of each Aurora member, which share many similarities, we investigated the expression profiles of the family in normal tissues and a panel of different phases of the HeLa cell cycle. Finally, both Aurora-A and -B were overexpressed in a majority of esophageal tumor tissues in comparison to the normal variants. Taken together, the results show that each Aurora member exhibits distinct expression patterns, implying that they are engaged in different biological processes to accomplish more elaborate cell physiological functions in higher organisms.
- Published
- 2018