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Overexpression of Hox transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is associated with tumorigenesis and multiple cancer types including lung cancer. In this study, the association between two HOTAIR single nucleotide polymorphisms (SNPs) (rs12826786 and rs1899663) on the risk and clinical characteristics of lung cancer in a Turkish population was investigated. We genotyped HOTAIR rs12826786 and rs1899663 polymorphisms in 180 Turkish people including 87 lung cancer patients (71 males and 16 females) and 93 age-matched healthy controls (67 males and 26 females) by a TaqMan real-time polymerase chain reaction method. The mean age value of the lung cancer patients and control subjects were 59.27 ± 10.55 and 61.77 ± 12.00, respectively. We found that none of the two HOTAIR polymorphisms (rs12826786 T>C, rs1899663A>C) has any significant association with the increased risk of lung cancer in any type of inheritance genetic models. However, our research indicated that carriers of Trs12826786/Crs1899663 (ht3) (P = 0.03) had an increased risk of lung cancer susceptibility.

The main aim of this study was to investigate the genotoxic effects of doripenem (DRP) using both cytogenetic and molecular test systems. Although there have been some studies reporting the effects of DRP, none of them has shown the genotoxic effects of DRP. In order to achieve the main aim of the study, the human peripheral lymphocytes were treated with 100 μg/ml, 200 μg/ml, and 400 μg/ml concentrations of DRP for 24 and 48 hours, and the chromosome aberration (CA) and micronucleus (MN) methods were used as the cytogenetic tests and RAPD-PCR method was used as the molecular test to determine the genotoxic effects of DRP. DRP did not induce the chromosome aberrations and micronucleus frequencies at all concentrations and at all treatment periods. So, it was concluded that DRP did not show any cytotoxic effect. However, DRP increased the number of polymorphic bands and decreased the ratio of genomic template stability, especially at the 48-hour treatment period. In this study, according to the obtained results, it was determined that DRP failed to show any genotoxic risk at the therapeutic doses. This result also indicates that DRP could be a reliable antibiotics according to its rapid metabolism.