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Objective: The aim of this study was to compare muscle strength, balance, and physical activity between children with chronic ankle instability and copers., Design: Cross-sectional study., Setting: Pediatric Physical Therapy laboratory., Participants: Children aged between 7 and 12 years old from regular schools who had a history of unilateral ankle sprain., Main Outcome Measures: Lower extremity strength, Static and dynamic standing balance, and Level of physical activity., Results: Fifteen children with chronic ankle instability and 15 copers were recruited. Demographic data showed no differences between group except for sex and the Cumberland Ankle Instability Tool-Youth (CAITY) score. Children with CAI had a significantly lower score during the standing long jump test (p < 0.05), poorer performance of single leg stance test (p < 0.001) and Y-Balance test in the anterior, posteromedial, and posterolateral directions (p < 0.05) when compared to copers. Total METs per week was significantly higher in the copers (p < 0.05)., Conclusion: Children with chronic ankle instability exhibited weaker lower limb strength, poorer static and dynamic standing balance, and participate in less physical activity compared to those in a coper group which may have future negative health consequences. Knowledge about the differences between the two groups may provide guidance for physical educators and physical therapists. (200 words)., Competing Interests: Declaration of competing interest There are no potential conflicts of interest to declare., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)