1. [Effect of RelB on HIV-1 Vpr-mediated transcription activation and cell G2/M arrest].
- Author
-
Liu RK, Gao Y, Lin YQ, Tan J, Geng YQ, and Qiao WT
- Subjects
- HIV Long Terminal Repeat, HeLa Cells, Humans, NF-kappa B genetics, Cell Cycle Checkpoints, Cell Division, G2 Phase, Transcription Factor RelB physiology, Transcriptional Activation, vpr Gene Products, Human Immunodeficiency Virus physiology
- Abstract
Vpr, an auxiliary protein of HIV-1(Human immunodeficiency virus type 1), exerts important functions to promote viral replication and AIDS progression. In this study, we performed a yeast two-hybrid screening assay using human cDNA library to further investigate the molecular mechanism of various functions of Vpr RelB, a key protein in NF-kappaB signaling pathway, was identified as a Vpr interaction protein by co-immunoprecipitation. Further investigations indicated that RelB not only promoted the Vpr-mediated activation of NF-kappaB reporter gene, but also enhanced the transactivation of HIV LTR. Moreover, the results showed that RelB promoted Vpr-induced cell cycle G2/M arrest. Collectively, these results indicated that RelB might interact with Vpr and regulate its transcriptional activation and cell cycle arrest.
- Published
- 2013