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The article presents a study which examines SYBR Green I real-time (RT) quantitative polymerase chain reaction (PCR) technique's development and application for avian metapneumovirus subgroup B strain. The study determined the sensitivity, reproducibility and specificity of the SYBR Green I fluorescence quantitative PCR standard curve established by recombinant plasmid. Results show that the standard curve provides a good linear relationship between templat concentration and threshold cycle.

The article presents a study which examines and identify one strain of tembusu virus obtained from infected duckling. Topics discussed in the study include the egg lethal dose50 (ELD50) of the virus, the virulency of the virus, and the cause of death of duck embryos affected by the virus.

The article focuses on a study related to the detection of Tembusu virus E gene from ducks by using loop-mediated isothermal amplification (LAMP) assay. Topics discussed include development of the assay using primers which targets the E gene of the virus, determination of the cross-reactivity with other viruses such as duck plagues virus, avian influenza virus, and Newcastle disease virus, and the significance of the LAMP assay in the molecular epidemiological investigation of the virus.

An abstract of the article "Preparation of digoxigenin-labeled DNA probe for detection of duck flavivirus and its application," by Gao Xu-Hui and colleagues is presented.

An abstract of the article "Development of a semi-nested RT-PCR assay for detection of duck flavivirus," by Tang Yi and colleagues is presented.