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Microbially induced calcium carbonate precipitation (MICP) is one widespread approach to soil clay improvement in sustainable biological engineering in the world. The green and efficient approach can also greatly contribute to a series of environmental and ecological challenges. Among them, urea-producing bacteria can be used to induce calcium carbonate precipitation for better soil consolidation. Bacterially generated urease enzymes can decompose the urea to further promote the binding of Ca2+ and CO32-. Therefore, the calcium carbonate precipitation can be expected to apply to desertification combating. However, the urea-producing bacteria can be limited to environmental adaptability and weak function in a harsh desert environment. Many technical bottlenecks are urgent issues for the sand-fixation using calcium carbonate precipitation induced by urea-producing bacteria. It is a high demand to develop native urea-producing bacteria. In the preliminary research, the strain of Sporosarcina pasteurii was identified from the aeolian soils in the Mu Us desert. In this research, the optimal reaction conditions were determined to verify the sand-fixation effect in the desert soil after optimization. A series of single-factor experiments were designed during 0-72 h with the different experimental gradients by pH (7, 8, 9, and 10), bacterial inoculum (1%, 2%, 3%, 4%, and 5%), the ratio of bacterial and cementing solution (25%, 50%, and 75%), and temperature (30, 35, and 40 ℃). The initial urease activity and calcium deposition rate were tested using the response surface method. The optimal reaction factors and conditions were then determined from the six factors (reaction time, pH, bacterial inoculum, the ratio of bacterial and cementing solution, reaction temperature, and initial urease activity). The sand-fixation experiments were carried out on the aeolian soil from the Mu Us desert under optimized conditions. Mineral morphologies and properties were then characterized by the scanning electron microscope (SEM). The thickness and hardness of the soil consolidated layer, soil particle distribution, soil organic matter and calcium carbonate content were measured to calculate the erodibility factor (EF) under soil wind erosion. The results showed that: 1) The main reaction drivers of calcium carbonate precipitation were determined as the reaction temperature, reaction time, pH value, as well as the ratio of bacterial and cementing solution. An optimal combination of conditions was achieved at 34.47 ℃, 71.68 h, pH 7.73, and 74.94% ratio. In this condition, the maximum rate of calcium deposition was 42.33%. 2) The calcium carbonate precipitation was obtained in the polycrystalline phase composition with the cubic, rhombic, spherical, and amorphous under the optimized conditions. Diverse calcium carbonate precipitation was formed on the surface of sand grains, or embedded in the sand grains. 3) Sand-fixation by induced calcium carbonate precipitation was significantly enhanced after optimization. The number of sand particles increased significantly (P<0.05), and the thickness and hardness of the soil consolidated layer increased by 5 and 10 times, respectively. The contents of organic matter and calcium carbonate increased significantly, whereas, the wind EF decreased from 0.94 to 0.66. Therefore, the urea-producing bacteria-induced calcium carbonate precipitation can serve as the new effective and favorable sand fixation for desertification combating in the future. It is still necessary to develop the distribution of simultaneous ecological restoration, in order to gradually promote this new technology in large-scale field experiments. Consequently, in the field application, the sand fixation effect can be enhanced to further determine the application time and water under suitable conditions and the soil compaction will be avoided by appropriately breaking. [ABSTRACT FROM AUTHOR]

Calcic vertisol is a typical kind of low-yield field with a total area of about 4 million hm2 in China. Calcareous concretion with particle size larger than 2 mm has been one of the representative characteristics of calcic vertisol. The limitation of soil available water is an important reason for crop failure in this region. Therefore, the curve of soil water retention dominates the estimation of available water content. However, most studies focused on the effects of calcareous concretion on soil water retention of calcic vertisol mainly under laboratory conditions. It is lacking that under field conditions. In this study, both field surveys and laboratory experiments were conducted to explore the effect of calcareous concretion distribution in 0-1 m soil profile on soil water retention. Firstly, the spatial distribution of calcareous concretion was investigated in the study plot with 150 m length, 50 m width, and 1 m depth. After then, soil profiles (0-1 m soil depth) were chosen to divide into 5 layers with a 20 cm interval. Disturbed and undisturbed soil samples were collected in each layer. Physicochemical properties were evaluated to measure the distribution of soil particle size, size and content of calcareous concretion, bulk densities of soil, and calcareous concretions. Meanwhile, the curves of soil water retention were determined using high speed centrifuge. A pressure plate method was conducted to measure water retention curves in calcareous concretion. The results showed that the mass contents of clay, silt, and sand were 30.56%-39.75%, 39.88%-45.34%, and 18.67%-24.45%, respectively, which belonged to silty clay soil in the classification standard of the United States Department of Agriculture (USDA). Clayey particles decreased, but sandy particles increased significantly with the increase of soil depth. Calcareous concretion was mainly distributed in the soil depth of >20-100 cm, where the content and size increased as soil depth increased. Calcareous concretions content in the surface soil (0-20 cm) was very low (with mass content 0.02%), where only a few calcareous concretions of 2-5 mm were discovered. The maximum content of calcareous concretion was detected at > 80-100 cm (up to 11.42%), where the calcareous concretion content was 8.04% in the size of >8-30 mm. Soil bulk density ranged from 1.23-1.61 g cm-3. The densities of calcareous concretions were 2.01, 2.21, and 2.23 g cm-3 with the size of 2-5 mm, >5-8 mm, and >8-30 mm, respectively. Soil bulk density increased along with the calcareous concretion content increasing. Water retention curves of soil and calcareous concretion were well fitted by the van Genuchten model, with the determination coefficient larger than 0.95. The saturated water content of surface soil (0-20 cm) was significantly higher than that of 20-100 cm soil. Nevertheless, the Gravitational-water content in the surface soil (0-20 cm) was much larger than that in the soil of > 20-100 cm, which was related to macropores formed during tillage. In the subsurface (>20-100 cm), soil gravitational-water content and available water-holding capacity increased with the increase of soil depth, but the field capacity and wilting point decreased. Calcareous concretion maintained a non-negligible amount of water, 0.25, 0.22, and 0.20 cm3 /cm3 in the particles with the size of 2-5 mm, >5-8 mm, and >8-30 mm, respectively. But the water-holding capacities of calcareous concretion were significantly lower than that of soil. The effects of calcareous concretion on water-holding capacity mainly occurred in the subsurface soil. Soil saturated water content, field capacity, and wilting point decreased with increasing calcareous concretion. Interestingly, calcareous concretion content was positively correlated with the soil sand content, but negatively correlated with the soil clay content. Calcareous concretion can bind soil particles together to prevent sand weathering, and thereby effectively improve soil texture. More calcareous concretion led to higher sand contents, and thus increased the available water-holding capacity in soil. This finding can provide a theoretical basis to accurately assess the soil water-holding capacity in calcic vertisol for precision irrigation and high crop productivity. [ABSTRACT FROM AUTHOR]

Rice-wheat rotation area, with heavy clay soil texture, that it is wheat planting area accounts for more than 37% of the total wheat sowing area in China as one of the major grain production bases, and rice-wheat rotation is the main cropping pattern in this region. Meanwhile, because of the shortage crop stubble and more rainfall in autumn, which lead to serious wet damage during the tilling and seeding stage for this field. Currently, most of the existing no-tillage and less-tillage seeding equipment for wheat sowing in rice stubble areas were optimized and developed on the basis of traditional contact-type wheat seeding technology, which lead to adhesion and serious blockage phenomenon, as well as increasing of operating resistance and power consumption during working. Therefore, a innovative technical proposal of non-contact type pneumatic seeding technology that could avoid contacting between sowing parts and soil was introduced in this paper, which in order to solve the problems of soil adhesion, blockage, drag increment as well as poor sowing quality for wheat seeders under the condition of sticky heavy loam clay in rice-wheat rotation areas. Experiment that utilized a self-made platform for wheat pneumatic seeding was conducted, which could rip wheat seed into the soil condition of clean seed bed without straw and stubble mulching by high pressure accelerating airflow. During the pneumatic seeding experiment, keeping a certain pneumatic seeding height HG and incident angle α between the accelerating tube and topsoil surface, high-pressure gas was injected from the upper apex of the air nozzle and ejected from the bottom of it, which a stable high-speed airflow was formed in the accelerator tube (the length of the accelerator tube is 600 mm and the diameter is 10 mm). Then wheat seeds were put into the seed entrance and absorbed at a high speed under the negative air pressure of the ejector, during which the rubber sleeve could protect wheat seeds from damaging. After mixing with airflow, wheat seeds were accelerated by high-speed air flow in the accelerating tube. Finally, the wheat seeds flew out and ripped into the soil at a certain incident angle and high pneumatic seeding velocity. At the same time, high-speed photography technology was used to study the parameters of pneumatic seeding velocity, vertical velocity and pneumatic seeding depth under six levels (15°, 30°, 45°, 60°, 75°, 90°) of incident angles, and pneumatic seeding experiments under five different accelerating air pressures of 0.2 MPa, 0.4 MPa, 0.6 MPa, 0.8 MPa and 1 MPa for each incident angle were carried out respectively. Pneumatic seeding experimental results showed that: For pneumatic seeding in soil without straw and stubble mulching, when the incident angle increases continuously, wheat seeds can achieve higher vertical component of pneumatic seeding velocity deeper and pneumatic seeding depth at the same accelerating air pressures, the probability of horizontal slip and bounce of wheat seeds after touching soil show an decreasing trend, and pneumatic seeding depth has a positive correlation with pneumatic seeding velocity under each incident angle. Moreover, wheat seeds show various pneumatic seeding depth and soil impact effect without damage after pneumatic seeding. When the incident angles and the vertical component of pneumatic seeding velocity were more than 45° and 25 m/s respectively, wheat seeds can be completely ripped into the soil and the emergence rate is more than 86%. The best pneumatic seeding effect achieved as incident angle is 90°. In order to realize wheat vertical pneumatic seeding in field operation, the horizontal component of pneumatic seeding velocity should be equal to the operating speed of seeder and the vertical component of pneumatic seeding velocity should be greater than 25 m/s, which the seeder operating speed shows positively and negatively correlated with pneumatic seeding velocity and incident angle, respectively. The experimental results can provide basic data and technical support for the development of non-contact wheat seeding equipment in rice-wheat rotation area. [ABSTRACT FROM AUTHOR]