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BACKGROUND: Most of the biomechanical studies on kyphosis have focused on trunk muscle strength and sagittal plane balance, and little has been reported on the biomechanical response within the spine during kyphosis. OBJECTIVE: To investigate the biomechanical response of the spine during postural kyphosis by simulating the process of postural kyphosis. METHODS: A three-dimensional finite element model of the normal thoracolumbar segment (T1-S1 segment) was established by using the finite element method. 10 groups of pure bending loads from 1.15-11.52 N·m were applied using a three-point force system on T1, T6, and T12 vertebrae to simulate the process of postural kyphosis in normal humans. The relationship between the loads and Cobb angle and the biomechanical responses of vertebrae, ribs, and intervertebral discs were analyzed. RESULTS AND CONCLUSION: (1) During postural kyphosis, the Cobb angle size of T1-T12 segments was linearly related to the load size. (2) The maximum stresses on the vertebrae, ribs, and intervertebral discs increased with increasing load. (3) Under the action of 11.52 N·m moment, the maximum stresses on the vertebral body, ribs, and intervertebral disc were found in the front of the T6 vertebral body, the rib head of the 10th pair of ribs, and the right posterior side of the intervertebral disc of the T5-T6 segments. (4) The results of this study suggest that postural kyphosis leads to increased stress on the vertebrae, ribs, and discs, with the most significant increase in stress on the anterior side of the T6 vertebrae, at the rib head of the 10th pair of ribs, and on the anterior side of the disc at the T5-T6 segment, as well as on the posterior side, which may increase the risk of injury to the vertebrae, ribs, and discs, which provides a biomechanical basis for the design of kyphosis orthoses. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Rheumatoid arthritis is an autoimmune disease. Research on rheumatoid arthritis mainly focuses on its pathogenesis and target therapy. OBJECTIVE: To search for differential genes by bioinformatics analysis of rheumatoid arthritis synovitis gene chip datasets, and to establish and improve the gene regulation network of rheumatoid arthritis. METHODS: GEO2R was used to analyze the differential expression of rheumatoid arthritis chips screened from the database. R language and DAVID were used for gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of differentially expressed genes. String database was used for protein-protein interaction network analysis. Cystoscape software was used to obtain key genes. qPCR and western blot were used for experimental verification of some key target genes. RESULTS AND CONCLUSION: There were 2 064 differentially expressed genes in the synovial tissue of patients with rheumatoid arthritis, including 625 upregulated genes and 1 439 down-regulated genes. Differentially expressed genes are mainly involved in immune response-activated cell surface receptor signaling pathway, antigen receptor mediated signaling pathway and other biological responses. Ten key target genes, including epidermal growth factor receptor (EGFR), MYC, RELA, ITGB2, LCK, EPHB2, IRF4, NRAS, FN1, and MAPK1, were identified by the protein-protein interaction network analysis. The number of protein interaction lines based on the protein-protein interaction network and Cystoscape algorithm score were used to obtain five genes with high significance, including EGFR, MYC, RELA, ITGB2, and LCK, and the experimental verification of these genes was carried out by qPCR and western blot experiments. The mRNA and protein expression levels of EGFR, MYC, RELA, ITGB2, and LCK genes in the synovial tissue of patients with rheumatoid arthritis were significantly higher than those of the control group, and the expression of EGFR changed most significantly. To conclude, there are distinct gene expression characteristics in the synovial tissue of knee joint after rheumatoid arthritis, and EGFR is the most valuable differentially expressed gene in the synovium of rheumatoid arthritis. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Due to the stability of the physical and chemical properties of zirconia ceramics, its poor adhesiveness has seriously affected the clinical application of zirconia ceramic prosthesis. OBJECTIVE: To analyze the effect of different surface treatment methods and resin cement on the bonding strength of zirconia ceramics. METHODS: Totally 240 zirconia blocks were randomly divided into 16 groups, with 15 in each group. In group A, the treatment method was sandblasting+hydrofluoric acid+Monobond-s+Panavia F 2.0. In group B, the treatment method was sandblasting+hydrofluoric acid+Monobond-s+Perma Cem-Dual. In group C, the treatment method was sandblasting+hydrofluoric acid+new silane coupling agent+Panavia F 2.0. In group D, the treatment method was sandblasting+hydrofluoric acid+new silane coupling agent+Perma Cem-Dual. In group E, the treatment method was sandblasting+Monobond-s+Panavia F 2.0. In group F, the treatment method was sandblasting+Monobond-s+Perma Cem-Dual. In group G, the treatment method was sandblasting+Monobond-s+Panavia F 2.0 resin cement binder. In group H, the treatment method was sandblasting+new silane coupling agent+Perma Cem-Dual. In group I, the treatment method was hydrofluoric acid+Monobond-s+Panavia F 2.0. In group J, the treatment method was hydrofluoric acid+Monobond-s+Perma Cem-Dual. In group K, the treatment method was hydrofluoric acid+new silane coupling agent+Panavia F 2.0. In group L, the treatment method was hydrofluoric acid+new silane coupling agent+Perma Cem-Dual. In group M, the treatment method was Monobond-s+Panavia F 2.0. In group N, the treatment method was Monobond-s+Perma Cem-Dual. In group O, the treatment method was new silane coupling agent+Panavia F 2.0. In group P, the treatment method was new silane coupling agent+Perma Cem-Dual. Panavia F 2.0 and Perma Cem-Dual were resin cement binders. Monobond-s was commercial silane coupling agent. The bonding strength of each group was tested. RESULTS AND CONCLUSION: (1) The bonding strength of groups C, G and H was higher than that of the remaining 13 groups (P < 0.05). The bonding strength of groups A, B, C, D, E, F, G and H was higher than that of groups I, J, K, L, M, N, and P (P < 0.05). (2) The results show that the combined use of zirconia sandblasting and new silane coupling agent can significantly improve the bonding strength of ceramic-cement. Hydrofluoric acid pretreatment is ineffective. The different types of cement will have a certain effect on the bonding strength. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)