1. Dissecting embryonic and extraembryonic lineage crosstalk with stem cell co-culture.
- Author
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Wei, Yulei, Zhang, E., Yu, Leqian, Ci, Baiquan, Sakurai, Masahiro, Guo, Lei, Zhang, Xin, Lin, Sirui, Takii, Shino, Liu, Lizhong, Liu, Jian, Schmitz, Daniel A., Su, Ting, Zhang, Junmei, Shen, Qiaoyan, Ding, Yi, Zhan, Linfeng, Sun, Hai-Xi, Zheng, Canbin, and Xu, Lin
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STEM cells , *EMBRYONIC stem cells , *KRA , *TROPHOBLAST , *EXTRACELLULAR matrix , *CELL culture , *FETAL tissues - Abstract
Embryogenesis necessitates harmonious coordination between embryonic and extraembryonic tissues. Although stem cells of both embryonic and extraembryonic origins have been generated, they are grown in different culture conditions. In this study, utilizing a unified culture condition that activates the FGF, TGF-β, and WNT pathways, we have successfully derived embryonic stem cells (FTW-ESCs), extraembryonic endoderm stem cells (FTW-XENs), and trophoblast stem cells (FTW-TSCs) from the three foundational tissues of mouse and cynomolgus monkey (Macaca fascicularis) blastocysts. This approach facilitates the co-culture of embryonic and extraembryonic stem cells, revealing a growth inhibition effect exerted by extraembryonic endoderm cells on pluripotent cells, partially through extracellular matrix signaling. Additionally, our cross-species analysis identified both shared and unique transcription factors and pathways regulating FTW-XENs. The embryonic and extraembryonic stem cell co-culture strategy offers promising avenues for developing more faithful embryo models and devising more developmentally pertinent differentiation protocols. [Display omitted] • A common culture condition for mouse and monkey embryonic and extraembryonic stem cells • Stem cell co-cultures reveal crosstalk between embryonic and extraembryonic lineages • Extraembryonic endoderm cells limit pluripotent cell growth via ECM signaling • Cross-species study uncovers common/unique genes regulating extraembryonic endoderm cells A unified culture condition for all three primary tissues of mouse and cynomolgus monkey blastocysts is established, facilitating stem cell co-culture experiments and uncovering intricate interactions between embryonic and extraembryonic lineages. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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