1. Protocol for rapid assessment of the efficacy of novel Wnt inhibitors using zebrafish models.
- Author
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Haney MG, Wimsett M, Liu C, and Blackburn JS
- Subjects
- Animals, Female, Male, Neoplasms, Experimental metabolism, Wnt Proteins metabolism, Zebrafish, Antineoplastic Agents pharmacology, Antineoplastic Agents toxicity, Drug Evaluation, Preclinical methods, Wnt Proteins antagonists & inhibitors, Wnt Signaling Pathway drug effects
- Abstract
Dysregulation of Wnt signaling is a hallmark of many cancers, and the development of effective, non-toxic small-molecule Wnt inhibitors is desirable. Off-target toxicities of new compounds are typically tested in mouse models, which is both costly and time consuming. Here, we present a rapid and inexpensive protocol to determine the in vivo toxicity and efficacy of novel Wnt inhibitors in zebrafish using a combination of a fluorescence reporter assay as well as eye rescue and fin regeneration assays. These experiments are completed within 1 week to rapidly narrow drug candidates before moving to more expensive pre-clinical testing. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020)., Competing Interests: The authors declare no competing interests., (© 2021 The Authors.)
- Published
- 2021
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