1. Protocol to create phenotypic primary human hepatocyte cultures using the RASTRUM 3D cell model platform.
- Author
-
Yee C, Chan YL, Utama R, Besnier M, Engel M, and Belfiore L
- Subjects
- Humans, Cell Culture Techniques methods, Troglitazone pharmacology, Phenotype, Acetaminophen pharmacology, Cells, Cultured, Cell Culture Techniques, Three Dimensional methods, Primary Cell Culture methods, Cell Survival drug effects, Hepatocytes cytology, Hepatocytes metabolism, Hepatocytes drug effects
- Abstract
To improve human hepatotoxicity prediction, in vitro liver cell models replicating hepatocyte function, drug metabolism, and toxicity are required. Here, we present a protocol for creating 3D primary human hepatocyte (PHH) cell models using the RASTRUM Platform. We describe the process for PHH model generation; procedures for characterizing the PHH model, including viability, albumin production, and CYP450 inducibility; and drug treatment using acetaminophen and troglitazone. This protocol has applications in upscaling phenotypic hepatotoxicity applications., Competing Interests: Declaration of interests C.Y., R.U., M.B., M.E., and L.B. are employees and shareholders of Inventia Life Science Pty. Ltd. Inventia has an interest in commercializing the RASTRUM Platform., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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