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The superior colliculus, or tectum in the case of non-mammalian vertebrates, is a part of the brain that registers events in the surrounding space, often through vision and hearing, but also through electrosensation, infrared detection, and other sensory modalities in diverse vertebrate lineages. This information is used to form maps of the surrounding space and the positions of different salient stimuli in relation to the individual. The sensory maps are arranged in layers with visual input in the uppermost layer, other senses in deeper positions, and a spatially aligned motor map in the deepest layer. Here, we will review the organization and intrinsic function of the tectum/superior colliculus and the information that is processed within tectal circuits. We will also discuss tectal/superior colliculus outputs that are conveyed directly to downstream motor circuits or via the thalamus to cortical areas to control various aspects of behavior. The tectum/superior colliculus is evolutionarily conserved among all vertebrates, but tailored to the sensory specialties of each lineage, and its roles have shifted with the emergence of the cerebral cortex in mammals. We will illustrate both the conserved and divergent properties of the tectum/superior colliculus through vertebrate evolution by comparing tectal processing in lampreys belonging to the oldest group of extant vertebrates, larval zebrafish, rodents, and other vertebrates including primates. Isa et al. review recent advances in understanding the sensory inputs, the integral circuitry and computations, and sensorimotor contributions of the tectum/superior colliculus. They highlight both the properties that are conserved and those that have become specialized in different vertebrate lineages. [ABSTRACT FROM AUTHOR]

Summary The tectum has long been known as a hub of visual processing, and recent studies have elucidated many of the circuit-level mechanisms by which tectal neurons filter visual information. Here, we use population-scale imaging of tectal neurons expressing a genetically encoded calcium indicator to characterize tectal responses to non-visual stimuli in zebrafish. We identify ensembles of neurons responsive to stimuli for each of three sensory modalities: vision, audition, and water flow sensation. These ensembles display consistently represented response profiles to our stimuli, and each has a preferred stimulus and salient feature to which it is most responsive. Each sensory modality drives a unique spatial profile of activity in the tectal neuropil, suggesting that the neuropil’s laminar structure functionally subserves multiple modalities. The positions of the responsive neurons in the periventricular layer are also distinct across modalities, and very few neurons are responsive to multiple modalities. The cells contributing to each ensemble are highly variable from trial to trial, but ensembles contain “cores” of reliably responsive cells, suggesting a mechanism whereby they could maintain consistency in reporting salient stimulus features while retaining flexibility to report on similar stimuli. Finally, we find that co-presentation of auditory or water flow stimuli suppress visual responses in the tectum. [ABSTRACT FROM AUTHOR]

Summary: The ability to stimulate select neurons in isolated tissue and in living animals is important for investigating their role in circuits and behavior. We show that the engineered light-gated ionotropic glutamate receptor (LiGluR), when introduced into neurons, enables remote control of their activity. Trains of action potentials are optimally evoked and extinguished by 380 nm and 500 nm light, respectively, while intermediate wavelengths provide graded control over the amplitude of depolarization. Light pulses of 1–5 ms in duration at ∼380 nm trigger precisely timed action potentials and EPSP-like responses or can evoke sustained depolarizations that persist for minutes in the dark until extinguished by a short pulse of ∼500 nm light. When introduced into sensory neurons in zebrafish larvae, activation of LiGluR reversibly blocks the escape response to touch. Our studies show that LiGluR provides robust control over neuronal activity, enabling the dissection and manipulation of neural circuitry in vivo. [Copyright &y& Elsevier]

Most animals have complex auditory systems that identify salient features of the acoustic landscape to direct appropriate responses. In fish, these features include the volume, frequency, complexity, and temporal structure of acoustic stimuli transmitted through water. Larval fish have simple brains compared to adults but swim freely and depend on sophisticated sensory processing for survival. 1–5 Zebrafish larvae, an important model for studying brain-wide neural networks, have thus far been found to possess a rudimentary auditory system, sensitive to a narrow range of frequencies and without evident sensitivity to acoustic features that are salient and ethologically important to adult fish. 6,7 Here, we have combined a novel method for delivering water-borne sounds, a diverse assembly of acoustic stimuli, and whole-brain calcium imaging to describe the responses of individual auditory-responsive neurons across the brains of zebrafish larvae. Our results reveal responses to frequencies ranging from 100 Hz to 4 kHz, with evidence of frequency discrimination from 100 Hz to 2.5 kHz. Frequency-selective neurons are located in numerous regions of the brain, and neurons responsive to the same frequency are spatially grouped in some regions. Using functional clustering, we identified categories of neurons that are selective for a single pure-tone frequency, white noise, the sharp onset of acoustic stimuli, and stimuli involving a gradual crescendo. These results suggest a more nuanced auditory system than has previously been described in larval fish and provide insights into how a young animal's auditory system can both function acutely and serve as the scaffold for a more complex adult system. [Display omitted] • Larval zebrafish have neural responses to auditory stimuli up to 4 kHz • Different frequencies elicit distinct responses for frequencies below 2.5 kHz • Responses distinguish pure versus complex stimuli and gradual versus sharp onset • Frequency-selective neurons are organized spatially but are not clearly tonotopic Larval fish are thought to have rudimentary auditory systems sensitive to low frequency. Using calcium imaging, Poulsen et al. show neural responses in zebrafish larvae up to 4 kHz, frequency discrimination up to 2.5 kHz, and unique signatures for simple and complex sounds. The particular responses are located in specific locations across the brain. [ABSTRACT FROM AUTHOR]

Summary The vestibular system, which reports on motion and gravity, is essential to postural control, balance, and egocentric representations of movement and space. The motion needed to stimulate the vestibular system complicates studying its circuitry, so we previously developed a method for fictive vestibular stimulation in zebrafish, using optical trapping to apply physical forces to the otoliths. Here, we combine this approach with whole-brain calcium imaging at cellular resolution, delivering a comprehensive map of the brain regions and cellular responses involved in basic vestibular processing. We find responses broadly distributed across the brain, with unique profiles of cellular responses and topography in each region. The most widespread and abundant responses involve excitation that is graded to the stimulus strength. Other responses, localized to the telencephalon and habenulae, show excitation that is only weakly correlated to stimulus strength and that is sensitive to weak stimuli. Finally, numerous brain regions contain neurons that are inhibited by vestibular stimuli, and these neurons are often tightly localized spatially within their regions. By exerting separate control over the left and right otoliths, we explore the laterality of brain-wide vestibular processing, distinguishing between neurons with unilateral and bilateral vestibular sensitivity and revealing patterns whereby conflicting signals from the ears mutually cancel. Our results confirm previously identified vestibular responses in specific regions of the larval zebrafish brain while revealing a broader and more extensive network of vestibular responsive neurons than has previously been described. This provides a departure point for more targeted studies of the underlying functional circuits. Highlights • Optical traps allow brain-wide imaging of vestibular activity with SPIM and GCaMP • Vestibular stimulation drives characteristic patterns of excitation and inhibition • Consistent spatial patterns exist within and across numerous brain regions • Signals from the two ears cancel if contradictory vestibular stimuli are presented Favre-Bulle, Vanwalleghem, et al. use optical trapping to stimulate the vestibular system in stationary larval zebrafish and then use SPIM microscopy and GCaMP to describe resulting activity across the brain. The result is a brain-wide atlas of vestibular processing at cellular resolution. [ABSTRACT FROM AUTHOR]

Summary Looming visual stimuli result in escape responses that are conserved from insects to humans. Despite their importance for survival, the circuits mediating visual startle have only recently been explored in vertebrates. Here we show that the zebrafish thalamus is a luminance detector critical to visual escape. Thalamic projection neurons deliver dim-specific information to the optic tectum, and ablations of these projections disrupt normal tectal responses to looms. Without this information, larvae are less likely to escape from dark looming stimuli and lose the ability to escape away from the source of the loom. Remarkably, when paired with an isoluminant loom stimulus to the opposite eye, dimming is sufficient to increase startle probability and to reverse the direction of the escape so that it is toward the loom. We suggest that bilateral comparisons of luminance, relayed from the thalamus to the tectum, facilitate escape responses and are essential for their directionality. [ABSTRACT FROM AUTHOR]

Summary Spontaneous patterns of activity in the developing visual system may play an important role in shaping the brain for function. During the period 4–9 dpf (days post-fertilization), larval zebrafish learn to hunt prey, a behavior that is critically dependent on the optic tectum. However, how spontaneous activity develops in the tectum over this period and the effect of visual experience are unknown. Here we performed two-photon calcium imaging of GCaMP6s zebrafish larvae at all days from 4 to 9 dpf. Using recently developed graph theoretic techniques, we found significant changes in both single-cell and population activity characteristics over development. In particular, we identified days 5–6 as a critical moment in the reorganization of the underlying functional network. Altering visual experience early in development altered the statistics of tectal activity, and dark rearing also caused a long-lasting deficit in the ability to capture prey. Thus, tectal development is shaped by both intrinsic factors and visual experience. [ABSTRACT FROM AUTHOR]

Tissue regeneration and functional restoration after injury are considered as stem- and progenitor-cell-driven processes. In the central nervous system, stem cell-driven repair is slow and problematic because function needs to be restored rapidly for vital tasks. In highly regenerative vertebrates, such as zebrafish, functional recovery is rapid, suggesting a capability for fast cell production and functional integration. Surprisingly, we found that migration of dormant "precursor neurons" to the injury site pioneers functional circuit regeneration after spinal cord injury and controls the subsequent stem-cell-driven repair response. Thus, the precursor neurons make do before the stem cells make new. Furthermore, RNA released from the dying or damaged cells at the site of injury acts as a signal to attract precursor neurons for repair. Taken together, our data demonstrate an unanticipated role of neuronal migration and RNA as drivers of neural repair., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2021 Elsevier Inc. All rights reserved.)