1. A heme-sensing mechanism in the translational regulation of mitochondrial cytochrome c oxidase biogenesis.
- Author
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Soto IC, Fontanesi F, Myers RS, Hamel P, and Barrientos A
- Subjects
- Amino Acid Motifs, Amino Acid Sequence, Electron Transport Complex IV genetics, Kinetics, Mitochondrial Turnover, Molecular Sequence Data, Mutation, Protein Binding, Protein Processing, Post-Translational, Recombinant Proteins biosynthesis, Recombinant Proteins chemistry, Recombinant Proteins genetics, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins genetics, Sequence Alignment, Transcription Factors genetics, Transcription Factors metabolism, Electron Transport Complex IV metabolism, Heme metabolism, Mitochondria enzymology, Saccharomyces cerevisiae Proteins metabolism
- Abstract
Heme plays fundamental roles as cofactor and signaling molecule in multiple pathways devoted to oxygen sensing and utilization in aerobic organisms. For cellular respiration, heme serves as a prosthetic group in electron transfer proteins and redox enzymes. Here we report that in the yeast Saccharomyces cerevisiae, a heme-sensing mechanism translationally controls the biogenesis of cytochrome c oxidase (COX), the terminal mitochondrial respiratory chain enzyme. We show that Mss51, a COX1 mRNA-specific translational activator and Cox1 chaperone, which coordinates Cox1 synthesis in mitoribosomes with its assembly in COX, is a heme-binding protein. Mss51 contains two heme regulatory motifs or Cys-Pro-X domains located in its N terminus. Using a combination of in vitro and in vivo approaches, we have demonstrated that these motifs are important for heme binding and efficient performance of Mss51 functions. We conclude that heme sensing by Mss51 regulates COX biogenesis and aerobic energy production., (Copyright © 2012 Elsevier Inc. All rights reserved.) more...
- Published
- 2012
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