19 results on '"Haas, Simon"'
Search Results
2. ATACing single cells with phages.
- Author
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Maschmeyer, Patrick and Haas, Simon
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MITOCHONDRIAL DNA , *PROTEIN expression , *CHROMATIN - Abstract
Fiskin et al. (2021) developed a "multi-omics" approach that integrates phage-displayed single-domain antibodies ("nanobodies") with the assay for transposase-accessible chromatin (PHAGE-ATAC) to simultaneously determine protein expression, chromatin accessibility, and mitochondrial DNA mutations (for clonal tracing) in single cells. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
3. The cycling and aging mouse female reproductive tract at single-cell resolution.
- Author
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Winkler, Ivana, Tolkachov, Alexander, Lammers, Fritjof, Lacour, Perrine, Daugelaite, Klaudija, Schneider, Nina, Koch, Marie-Luise, Panten, Jasper, Grünschläger, Florian, Poth, Tanja, Ávila, Bianca Machado de, Schneider, Augusto, Haas, Simon, Odom, Duncan T., and Gonçalves, Ângela
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GENITALIA , *FEMALE reproductive organs , *SEXUAL cycle , *ESTRUS , *AGING - Abstract
The female reproductive tract (FRT) undergoes extensive remodeling during reproductive cycling. This recurrent remodeling and how it shapes organ-specific aging remains poorly explored. Using single-cell and spatial transcriptomics, we systematically characterized morphological and gene expression changes occurring in ovary, oviduct, uterus, cervix, and vagina at each phase of the mouse estrous cycle, during decidualization, and into aging. These analyses reveal that fibroblasts play central—and highly organ-specific—roles in FRT remodeling by orchestrating extracellular matrix (ECM) reorganization and inflammation. Our results suggest a model wherein recurrent FRT remodeling over reproductive lifespan drives the gradual, age-related development of fibrosis and chronic inflammation. This hypothesis was directly tested using chemical ablation of cycling, which reduced fibrotic accumulation during aging. Our atlas provides extensive detail into how estrus, pregnancy, and aging shape the organs of the female reproductive tract and reveals the unexpected cost of the recurrent remodeling required for reproduction. [Display omitted] • Spatial and single-cell study of the cycling and aging female reproductive tract • Inflammation and ECM are recurrently modulated in the cycling female reproductive tract • Fibroblast control of inflammation and ECM remodeling is highly organ-specific • Fibrosis results from incomplete resolution of cyclic organ remodeling A spatial and single-cell atlas of the mouse female reproductive tract details transcriptional and cellular abundance changes occurring across estrous cycling, pregnancy, and aging, revealing how cycling-related remodeling contributes to inflammation and fibrotic development. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. Myc Depletion Induces a Pluripotent Dormant State Mimicking Diapause.
- Author
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Scognamiglio, Roberta, Cabezas-Wallscheid, Nina, Thier, Marc Christian, Altamura, Sandro, Reyes, Alejandro, Prendergast, Áine M., Baumgärtner, Daniel, Carnevalli, Larissa S., Atzberger, Ann, Haas, Simon, von Paleske, Lisa, Boroviak, Thorsten, Wörsdörfer, Philipp, Essers, Marieke A.G., Kloz, Ulrich, Eisenman, Robert N., Edenhofer, Frank, Bertone, Paul, Huber, Wolfgang, and van der Hoeven, Franciscus
- Subjects
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MYC oncogenes , *PLURIPOTENT stem cells , *DORMANCY (Biology) , *DELAYED implantation , *MICE genetics , *STEM cell treatment - Abstract
Summary Mouse embryonic stem cells (ESCs) are maintained in a naive ground state of pluripotency in the presence of MEK and GSK3 inhibitors. Here, we show that ground-state ESCs express low Myc levels. Deletion of both c-myc and N-myc ( dKO ) or pharmacological inhibition of Myc activity strongly decreases transcription, splicing, and protein synthesis, leading to proliferation arrest. This process is reversible and occurs without affecting pluripotency, suggesting that Myc-depleted stem cells enter a state of dormancy similar to embryonic diapause. Indeed, c-Myc is depleted in diapaused blastocysts, and the differential expression signatures of dKO ESCs and diapaused epiblasts are remarkably similar. Following Myc inhibition, pre-implantation blastocysts enter biosynthetic dormancy but can progress through their normal developmental program after transfer into pseudo-pregnant recipients. Our study shows that Myc controls the biosynthetic machinery of stem cells without affecting their potency, thus regulating their entry and exit from the dormant state. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
5. Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors.
- Author
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Paul, Franziska, Arkin, Ya’ara, Giladi, Amir, Jaitin, Diego Adhemar, Kenigsberg, Ephraim, Keren-Shaul, Hadas, Winter, Deborah, Lara-Astiaso, David, Gury, Meital, Weiner, Assaf, David, Eyal, Cohen, Nadav, Lauridsen, Felicia Kathrine Bratt, Haas, Simon, Schlitzer, Andreas, Mildner, Alexander, Ginhoux, Florent, Jung, Steffen, Trumpp, Andreas, and Porse, Bo Torben
- Subjects
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HETEROGENEITY , *BONE marrow physiology , *GENE regulatory networks , *HEMATOPOIETIC system , *HEMATOPOIESIS - Abstract
Summary Within the bone marrow, stem cells differentiate and give rise to diverse blood cell types and functions. Currently, hematopoietic progenitors are defined using surface markers combined with functional assays that are not directly linked with in vivo differentiation potential or gene regulatory mechanisms. Here, we comprehensively map myeloid progenitor subpopulations by transcriptional sorting of single cells from the bone marrow. We describe multiple progenitor subgroups, showing unexpected transcriptional priming toward seven differentiation fates but no progenitors with a mixed state. Transcriptional differentiation is correlated with combinations of known and previously undefined transcription factors, suggesting that the process is tightly regulated. Histone maps and knockout assays are consistent with early transcriptional priming, while traditional transplantation experiments suggest that in vivo priming may still allow for plasticity given strong perturbations. These data establish a reference model and general framework for studying hematopoiesis at single-cell resolution. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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6. Braveheart, a Long Noncoding RNA Required for Cardiovascular Lineage Commitment
- Author
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Klattenhoff, Carla A., Scheuermann, Johanna C., Surface, Lauren E., Bradley, Robert K., Fields, Paul A., Steinhauser, Matthew L., Ding, Huiming, Butty, Vincent L., Torrey, Lillian, Haas, Simon, Abo, Ryan, Tabebordbar, Mohammadsharif, Lee, Richard T., Burge, Christopher B., and Boyer, Laurie A.
- Subjects
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NON-coding RNA , *GENE expression , *EMBRYONIC stem cells , *CELL differentiation , *ANIMAL epigenetics , *GENE regulatory networks , *HEART cells - Abstract
Summary: Long noncoding RNAs (lncRNAs) are often expressed in a development-specific manner, yet little is known about their roles in lineage commitment. Here, we identified Braveheart (Bvht), a heart-associated lncRNA in mouse. Using multiple embryonic stem cell (ESC) differentiation strategies, we show that Bvht is required for progression of nascent mesoderm toward a cardiac fate. We find that Bvht is necessary for activation of a core cardiovascular gene network and functions upstream of mesoderm posterior 1 (MesP1), a master regulator of a common multipotent cardiovascular progenitor. We also show that Bvht interacts with SUZ12, a component of polycomb-repressive complex 2 (PRC2), during cardiomyocyte differentiation, suggesting that Bvht mediates epigenetic regulation of cardiac commitment. Finally, we demonstrate a role for Bvht in maintaining cardiac fate in neonatal cardiomyocytes. Together, our work provides evidence for a long noncoding RNA with critical roles in the establishment of the cardiovascular lineage during mammalian development. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
7. Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors.
- Author
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Paul, Franziska, Arkin, Ya’ara, Giladi, Amir, Jaitin, Diego Adhemar, Kenigsberg, Ephraim, Keren-Shaul, Hadas, Winter, Deborah, Lara-Astiaso, David, Gury, Meital, Weiner, Assaf, David, Eyal, Cohen, Nadav, Lauridsen, Felicia Kathrine Bratt, Haas, Simon, Schlitzer, Andreas, Mildner, Alexander, Ginhoux, Florent, Jung, Steffen, Trumpp, Andreas, and Porse, Bo Torben
- Subjects
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PROGENITOR cells , *CELL lines , *GENETIC transcription , *MYELOID leukemia , *CYTOLOGICAL research - Published
- 2016
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8. Aged intestinal stem cells propagate cell-intrinsic sources of inflammaging in mice.
- Author
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Funk MC, Gleixner JG, Heigwer F, Vonficht D, Valentini E, Aydin Z, Tonin E, Del Prete S, Mahara S, Throm Y, Hetzer J, Heide D, Stegle O, Odom DT, Feldmann A, Haas S, Heikenwalder M, and Boutros M
- Subjects
- Mice, Animals, Stem Cells, Phenotype, Inflammation, Intestines, Intestinal Mucosa
- Abstract
Low-grade chronic inflammation is a hallmark of ageing, associated with impaired tissue function and disease development. However, how cell-intrinsic and -extrinsic factors collectively establish this phenotype, termed inflammaging, remains poorly understood. We addressed this question in the mouse intestinal epithelium, using mouse organoid cultures to dissect stem cell-intrinsic and -extrinsic sources of inflammaging. At the single-cell level, we found that inflammaging is established differently along the crypt-villus axis, with aged intestinal stem cells (ISCs) strongly upregulating major histocompatibility complex class II (MHC-II) genes. Importantly, the inflammaging phenotype was stably propagated by aged ISCs in organoid cultures and associated with increased chromatin accessibility at inflammation-associated loci in vivo and ex vivo, indicating cell-intrinsic inflammatory memory. Mechanistically, we show that the expression of inflammatory genes is dependent on STAT1 signaling. Together, our data identify that intestinal inflammaging in mice is promoted by a cell-intrinsic mechanism, stably propagated by ISCs, and associated with a disbalance in immune homeostasis., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
9. Resistance to mesenchymal reprogramming sustains clonal propagation in metastatic breast cancer.
- Author
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Saini M, Schmidleitner L, Moreno HD, Donato E, Falcone M, Bartsch JM, Klein C, Vogel V, Würth R, Pfarr N, Espinet E, Lehmann M, Königshoff M, Reitberger M, Haas S, Graf E, Schwarzmayr T, Strom TM, Spaich S, Sütterlin M, Schneeweiss A, Weichert W, Schotta G, Reichert M, Aceto N, Sprick MR, Trumpp A, and Scheel CH
- Subjects
- Humans, Female, Epithelial Cell Adhesion Molecule, Cell Line, Tumor, Breast metabolism, Clone Cells metabolism, Epithelial-Mesenchymal Transition, Breast Neoplasms pathology
- Abstract
The acquisition of mesenchymal traits is considered a hallmark of breast cancer progression. However, the functional relevance of epithelial-to-mesenchymal transition (EMT) remains controversial and context dependent. Here, we isolate epithelial and mesenchymal populations from human breast cancer metastatic biopsies and assess their functional potential in vivo. Strikingly, progressively decreasing epithelial cell adhesion molecule (EPCAM) levels correlate with declining disease propagation. Mechanistically, we find that persistent EPCAM expression marks epithelial clones that resist EMT induction and propagate competitively. In contrast, loss of EPCAM defines clones arrested in a mesenchymal state, with concomitant suppression of tumorigenicity and metastatic potential. This dichotomy results from distinct clonal trajectories impacting global epigenetic programs that are determined by the interplay between human ZEB1 and its target GRHL2. Collectively, our results indicate that susceptibility to irreversible EMT restrains clonal propagation, whereas resistance to mesenchymal reprogramming sustains disease spread in multiple models of human metastatic breast cancer, including patient-derived cells in vivo., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
10. Clonally resolved single-cell multi-omics identifies routes of cellular differentiation in acute myeloid leukemia.
- Author
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Beneyto-Calabuig S, Merbach AK, Kniffka JA, Antes M, Szu-Tu C, Rohde C, Waclawiczek A, Stelmach P, Gräßle S, Pervan P, Janssen M, Landry JJM, Benes V, Jauch A, Brough M, Bauer M, Besenbeck B, Felden J, Bäumer S, Hundemer M, Sauer T, Pabst C, Wickenhauser C, Angenendt L, Schliemann C, Trumpp A, Haas S, Scherer M, Raffel S, Müller-Tidow C, and Velten L
- Subjects
- Humans, Cell Differentiation, Neoplastic Stem Cells metabolism, Multiomics, Leukemia, Myeloid, Acute genetics
- Abstract
Inter-patient variability and the similarity of healthy and leukemic stem cells (LSCs) have impeded the characterization of LSCs in acute myeloid leukemia (AML) and their differentiation landscape. Here, we introduce CloneTracer, a novel method that adds clonal resolution to single-cell RNA-seq datasets. Applied to samples from 19 AML patients, CloneTracer revealed routes of leukemic differentiation. Although residual healthy and preleukemic cells dominated the dormant stem cell compartment, active LSCs resembled their healthy counterpart and retained erythroid capacity. By contrast, downstream myeloid progenitors constituted a highly aberrant, disease-defining compartment: their gene expression and differentiation state affected both the chemotherapy response and leukemia's ability to differentiate into transcriptomically normal monocytes. Finally, we demonstrated the potential of CloneTracer to identify surface markers misregulated specifically in leukemic cells. Taken together, CloneTracer reveals a differentiation landscape that mimics its healthy counterpart and may determine biology and therapy response in AML., Competing Interests: Declaration of interests The Department of Medicine V (Director C.M.-T.) receives research funding from multiple pharmaceutical and biotech companies especially for clinical trials but also for translational research., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
11. Cellular hierarchies predict drug response in acute myeloid leukemia.
- Author
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Raffel S, Velten L, and Haas S
- Subjects
- Humans, Neoplastic Stem Cells, Leukemia, Myeloid, Acute drug therapy, Leukemia, Myeloid, Acute genetics
- Abstract
In a recent Nature Medicine study, Zeng and colleagues integrate both genomic and stem cell models of acute myeloid leukemia (AML) by deconvoluting cellular hierarchies of more than 1,000 AML samples. This work introduces a framework capable of predicting drug responses to targeted therapies in future clinical trials., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
12. Inflammatory exposure drives long-lived impairment of hematopoietic stem cell self-renewal activity and accelerated aging.
- Author
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Bogeska R, Mikecin AM, Kaschutnig P, Fawaz M, Büchler-Schäff M, Le D, Ganuza M, Vollmer A, Paffenholz SV, Asada N, Rodriguez-Correa E, Frauhammer F, Buettner F, Ball M, Knoch J, Stäble S, Walter D, Petri A, Carreño-Gonzalez MJ, Wagner V, Brors B, Haas S, Lipka DB, Essers MAG, Weru V, Holland-Letz T, Mallm JP, Rippe K, Krämer S, Schlesner M, McKinney Freeman S, Florian MC, King KY, Frenette PS, Rieger MA, and Milsom MD
- Subjects
- Aged, Aging, Animals, Bone Marrow, Humans, Inflammation, Mice, Hematopoiesis, Hematopoietic Stem Cells
- Abstract
Hematopoietic stem cells (HSCs) mediate regeneration of the hematopoietic system following injury, such as following infection or inflammation. These challenges impair HSC function, but whether this functional impairment extends beyond the duration of inflammatory exposure is unknown. Unexpectedly, we observed an irreversible depletion of functional HSCs following challenge with inflammation or bacterial infection, with no evidence of any recovery up to 1 year afterward. HSCs from challenged mice demonstrated multiple cellular and molecular features of accelerated aging and developed clinically relevant blood and bone marrow phenotypes not normally observed in aged laboratory mice but commonly seen in elderly humans. In vivo HSC self-renewal divisions were absent or extremely rare during both challenge and recovery periods. The progressive, irreversible attrition of HSC function demonstrates that temporally discrete inflammatory events elicit a cumulative inhibitory effect on HSCs. This work positions early/mid-life inflammation as a mediator of lifelong defects in tissue maintenance and regeneration., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
13. Antigen presentation safeguards the integrity of the hematopoietic stem cell pool.
- Author
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Hernández-Malmierca P, Vonficht D, Schnell A, Uckelmann HJ, Bollhagen A, Mahmoud MAA, Landua SL, van der Salm E, Trautmann CL, Raffel S, Grünschläger F, Lutz R, Ghosh M, Renders S, Correia N, Donato E, Dixon KO, Hirche C, Andresen C, Robens C, Werner PS, Boch T, Eisel D, Osen W, Pilz F, Przybylla A, Klein C, Buchholz F, Milsom MD, Essers MAG, Eichmüller SB, Hofmann WK, Nowak D, Hübschmann D, Hundemer M, Thiede C, Bullinger L, Müller-Tidow C, Armstrong SA, Trumpp A, Kuchroo VK, and Haas S
- Subjects
- Cell Differentiation, T-Lymphocytes, Antigen Presentation, Hematopoietic Stem Cells
- Abstract
Hematopoietic stem and progenitor cells (HSPCs) are responsible for the production of blood and immune cells. Throughout life, HSPCs acquire oncogenic aberrations that can cause hematological cancers. Although molecular programs maintaining stem cell integrity have been identified, safety mechanisms eliminating malignant HSPCs from the stem cell pool remain poorly characterized. Here, we show that HSPCs constitutively present antigens via major histocompatibility complex class II. The presentation of immunogenic antigens, as occurring during malignant transformation, triggers bidirectional interactions between HSPCs and antigen-specific CD4
+ T cells, causing stem cell proliferation, differentiation, and specific exhaustion of aberrant HSPCs. This immunosurveillance mechanism effectively eliminates transformed HSPCs from the hematopoietic system, thereby preventing leukemia onset. Together, our data reveal a bidirectional interaction between HSPCs and CD4+ T cells, demonstrating that HSPCs are not only passive receivers of immunological signals but also actively engage in adaptive immune responses to safeguard the integrity of the stem cell pool., Competing Interests: Declaration of interests V.K.K. is a cofounder, has ownership interest, and is on the SAB of Celsius Therapeutics and Tizona Therapeutics., (Copyright © 2022 Elsevier Inc. All rights reserved.)- Published
- 2022
- Full Text
- View/download PDF
14. Decline in IGF1 in the bone marrow microenvironment initiates hematopoietic stem cell aging.
- Author
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Young K, Eudy E, Bell R, Loberg MA, Stearns T, Sharma D, Velten L, Haas S, Filippi MD, and Trowbridge JJ
- Subjects
- Aging, Animals, Cross-Sectional Studies, Hematopoiesis, Hematopoietic Stem Cells, Mice, Bone Marrow, Stem Cell Niche
- Abstract
Decline in hematopoietic stem cell (HSC) function with age underlies limited health span of our blood and immune systems. In order to preserve health into older age, it is necessary to understand the nature and timing of initiating events that cause HSC aging. By performing a cross-sectional study in mice, we discover that hallmarks of aging in HSCs and hematopoiesis begin to accumulate by middle age and that the bone marrow (BM) microenvironment at middle age induces and is indispensable for hematopoietic aging. Using unbiased approaches, we find that decreased levels of the longevity-associated molecule IGF1 in the local middle-aged BM microenvironment are a factor causing HSC aging. Direct stimulation of middle-aged HSCs with IGF1 rescues molecular and functional hallmarks of aging, including restored mitochondrial activity. Thus, although decline in IGF1 supports longevity, our work indicates that this also compromises HSC function and limits hematopoietic health span., Competing Interests: Declaration of interests J.J.T. holds a sponsored research project with H3 Biomedicine., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
15. Causes and Consequences of Hematopoietic Stem Cell Heterogeneity.
- Author
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Haas S, Trumpp A, and Milsom MD
- Subjects
- Animals, Hematopoietic Stem Cells metabolism, Humans, Hematopoietic Stem Cells cytology
- Abstract
Blood and immune cells derive from multipotent hematopoietic stem cells (HSCs). Classically, stem and progenitor populations have been considered discrete homogeneous populations. However, recent technological advances have revealed significant HSC heterogeneity, with evidence for early HSC lineage segregation and the presence of lineage-biased HSCs and lineage-restricted progenitors within the HSC compartment. These and other findings challenge many aspects of the classical view of HSC biology. We review the most recent findings regarding the causes and consequences of HSC heterogeneity, discuss their far-reaching implications, and suggest that so-called continuum-based models may help consolidate apparently divergent experimental observations in this field., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
16. An Intrinsic Interferon Program Protects Stem Cells from Viral Infection.
- Author
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Haas S and Trumpp A
- Subjects
- Humans, Interferons, Stem Cells
- Abstract
Specific protection mechanisms are required to safeguard stem cell integrity. In a recent issue of Cell, Wu et al. (2018) show that stem cells are equipped with high intrinsic expression of interferon-stimulated genes (ISGs) but remain refractory to acute interferon signaling. This protects stem cells from viral infection., (Copyright © 2018. Published by Elsevier Inc.)
- Published
- 2018
- Full Text
- View/download PDF
17. Systemic Virus Infections Differentially Modulate Cell Cycle State and Functionality of Long-Term Hematopoietic Stem Cells In Vivo.
- Author
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Hirche C, Frenz T, Haas SF, Döring M, Borst K, Tegtmeyer PK, Brizic I, Jordan S, Keyser K, Chhatbar C, Pronk E, Lin S, Messerle M, Jonjic S, Falk CS, Trumpp A, Essers MAG, and Kalinke U
- Subjects
- Animals, Cell Cycle, Cell Proliferation, Hematopoietic Stem Cells cytology, Mice, Signal Transduction, Hematopoietic Stem Cells metabolism, Infections virology
- Abstract
Quiescent long-term hematopoietic stem cells (LT-HSCs) are efficiently activated by type I interferon (IFN-I). However, this effect remains poorly investigated in the context of IFN-I-inducing virus infections. Here we report that both vesicular stomatitis virus (VSV) and murine cytomegalovirus (MCMV) infection induce LT-HSC activation that substantially differs from the effects triggered upon injection of synthetic IFN-I-inducing agents. In both infections, inflammatory responses had to exceed local thresholds within the bone marrow to confer LT-HSC cell cycle entry, and IFN-I receptor triggering was not critical for this activation. After resolution of acute MCMV infection, LT-HSCs returned to phenotypic quiescence. However, non-acute MCMV infection induced a sustained inflammatory milieu within the bone marrow that was associated with long-lasting impairment of LT-HSC function. In conclusion, our results show that systemic virus infections fundamentally affect LT-HSCs and that also non-acute inflammatory stimuli in bone marrow donors can affect the reconstitution potential of bone marrow transplants., (Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
18. Inflammation-Induced Emergency Megakaryopoiesis Driven by Hematopoietic Stem Cell-like Megakaryocyte Progenitors.
- Author
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Haas S, Hansson J, Klimmeck D, Loeffler D, Velten L, Uckelmann H, Wurzer S, Prendergast ÁM, Schnell A, Hexel K, Santarella-Mellwig R, Blaszkiewicz S, Kuck A, Geiger H, Milsom MD, Steinmetz LM, Schroeder T, Trumpp A, Krijgsveld J, and Essers MA
- Subjects
- Animals, Blood Platelets physiology, Cell Lineage, Cell Proliferation, Hematopoietic Stem Cells pathology, Hematopoietic Stem Cells physiology, Megakaryocyte Progenitor Cells physiology, Mice, Blood Platelets pathology, Inflammation pathology, Megakaryocyte Progenitor Cells pathology, Thrombopoiesis
- Abstract
Infections are associated with extensive platelet consumption, representing a high risk for health. However, the mechanism coordinating the rapid regeneration of the platelet pool during such stress conditions remains unclear. Here, we report that the phenotypic hematopoietic stem cell (HSC) compartment contains stem-like megakaryocyte-committed progenitors (SL-MkPs), a cell population that shares many features with multipotent HSCs and serves as a lineage-restricted emergency pool for inflammatory insults. During homeostasis, SL-MkPs are maintained in a primed but quiescent state, thus contributing little to steady-state megakaryopoiesis. Even though lineage-specific megakaryocyte transcripts are expressed, protein synthesis is suppressed. In response to acute inflammation, SL-MkPs become activated, resulting in megakaryocyte protein production from pre-existing transcripts and a maturation of SL-MkPs and other megakaryocyte progenitors. This results in an efficient replenishment of platelets that are lost during inflammatory insult. Thus, our study reveals an emergency machinery that counteracts life-threatening platelet depletions during acute inflammation., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
19. Identification of regulatory networks in HSCs and their immediate progeny via integrated proteome, transcriptome, and DNA methylome analysis.
- Author
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Cabezas-Wallscheid N, Klimmeck D, Hansson J, Lipka DB, Reyes A, Wang Q, Weichenhan D, Lier A, von Paleske L, Renders S, Wünsche P, Zeisberger P, Brocks D, Gu L, Herrmann C, Haas S, Essers MAG, Brors B, Eils R, Huber W, Milsom MD, Plass C, Krijgsveld J, and Trumpp A
- Subjects
- Adult, Cell Differentiation genetics, Cell Lineage genetics, Cluster Analysis, Epigenesis, Genetic, Gene Expression Profiling, Genome, Human genetics, Genomic Imprinting, Hematopoietic Stem Cells cytology, Humans, Molecular Sequence Data, Protein Isoforms genetics, Protein Isoforms metabolism, RNA, Long Noncoding genetics, Transcription Factors genetics, Transcription Factors metabolism, DNA Methylation genetics, Gene Regulatory Networks, Hematopoietic Stem Cells metabolism, Proteome metabolism, Transcriptome genetics
- Abstract
In this study, we present integrated quantitative proteome, transcriptome, and methylome analyses of hematopoietic stem cells (HSCs) and four multipotent progenitor (MPP) populations. From the characterization of more than 6,000 proteins, 27,000 transcripts, and 15,000 differentially methylated regions (DMRs), we identified coordinated changes associated with early differentiation steps. DMRs show continuous gain or loss of methylation during differentiation, and the overall change in DNA methylation correlates inversely with gene expression at key loci. Our data reveal the differential expression landscape of 493 transcription factors and 682 lncRNAs and highlight specific expression clusters operating in HSCs. We also found an unexpectedly dynamic pattern of transcript isoform regulation, suggesting a critical regulatory role during HSC differentiation, and a cell cycle/DNA repair signature associated with multipotency in MPP2 cells. This study provides a comprehensive genome-wide resource for the functional exploration of molecular, cellular, and epigenetic regulation at the top of the hematopoietic hierarchy., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
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