1. 11 C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography.
- Author
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Ozerskaya AV, Zamay TN, Kolovskaya OS, Tokarev NA, Belugin KV, Chanchikova NG, Badmaev ON, Zamay GS, Shchugoreva IA, Moryachkov RV, Zabluda VN, Khorzhevskii VA, Shepelevich N, Gappoev SV, Karlova EA, Saveleva AS, Volzhentsev AA, Blagodatova AN, Lukyanenko KA, Veprintsev DV, Smolyarova TE, Tomilin FN, Zamay SS, Silnikov VN, Berezovski MV, and Kichkailo AS
- Abstract
Identification of primary tumors and metastasis sites is an essential step in cancer diagnostics and the following treatment. Positron emission tomography-computed tomography (PET/CT) is one of the most reliable methods for scanning the whole organism for malignancies. In this work, we synthesized an
11 C-labeled oligonucleotide primer and hybridized it to an anti-cancer DNA aptamer. The11 C-aptamer was applied for in vivo imaging of Ehrlich ascites carcinoma and its metastases in mice using PET/CT. The imaging experiments with the11 C-aptamer determined very small primary and secondary tumors of 3 mm2 and less. We also compared11 C imaging with the standard radiotracer, 2-deoxy-2-[fluorine-18]fluoro-D-glucose (18 F-FDG), and found better selectivity of the11 C-aptamer to metastatic lesions in the metabolically active organs than18 F-FDG.11 C radionuclide with an ultra-short (20.38 min) half-life is considered safest for PET/CT imaging and does not cause false-positive results in heart imaging. Its combination with aptamers gives us high-specificity and high-contrast imaging of cancer cells and can be applied for PET/CT-guided drug delivery in cancer therapies., Competing Interests: Authors declare no competing interests., (© 2021 The Author(s).)- Published
- 2021
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