1. Contrasting somatic mutation patterns in aging human neurons and oligodendrocytes.
- Author
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Ganz J, Luquette LJ, Bizzotto S, Miller MB, Zhou Z, Bohrson CL, Jin H, Tran AV, Viswanadham VV, McDonough G, Brown K, Chahine Y, Chhouk B, Galor A, Park PJ, and Walsh CA
- Subjects
- Humans, Chromatin genetics, Chromatin metabolism, Mutation, Single-Cell Gene Expression Analysis, Whole Genome Sequencing, Polymorphism, Single Nucleotide, INDEL Mutation, Biological Specimen Banks, Oligodendrocyte Precursor Cells metabolism, Oligodendrocyte Precursor Cells pathology, Aging genetics, Aging pathology, Neurons metabolism, Neurons pathology, Oligodendroglia metabolism, Oligodendroglia pathology, Brain metabolism, Brain pathology
- Abstract
Characterizing somatic mutations in the brain is important for disentangling the complex mechanisms of aging, yet little is known about mutational patterns in different brain cell types. Here, we performed whole-genome sequencing (WGS) of 86 single oligodendrocytes, 20 mixed glia, and 56 single neurons from neurotypical individuals spanning 0.4-104 years of age and identified >92,000 somatic single-nucleotide variants (sSNVs) and small insertions/deletions (indels). Although both cell types accumulate somatic mutations linearly with age, oligodendrocytes accumulated sSNVs 81% faster than neurons and indels 28% slower than neurons. Correlation of mutations with single-nucleus RNA profiles and chromatin accessibility from the same brains revealed that oligodendrocyte mutations are enriched in inactive genomic regions and are distributed across the genome similarly to mutations in brain cancers. In contrast, neuronal mutations are enriched in open, transcriptionally active chromatin. These stark differences suggest an assortment of active mutagenic processes in oligodendrocytes and neurons., Competing Interests: Declaration of interests J.G. is now a Merck Research Laboratories (MRL) employee, and no work related to this manuscript was performed at MRL. P.J.P. is a member of the scientific advisory board for Bioskryb Genomics, Inc., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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