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Your search keyword '"Ruihua Fan"' showing total 2 results
Start Over Author "Ruihua Fan" Publisher cell physiol biochem press gmbh & co kg
2 results on '"Ruihua Fan"'

1. tRNA-Derived Fragments as Novel Predictive Biomarkers for Trastuzumab-Resistant Breast Cancer

Author

Chunxiao Sun, Fan Yang, Yanhong Zhang, Jiahui Chu, Jian Wang, Yifan Wang, Yanqiu Zhang, Jun Li, Yongfei Li, Ruihua Fan, Wei Li, Xiang Huang, Hao Wu, Ziyi Fu, Zefei Jiang, and Yongmei Yin

Subjects
Breast cancer, Trastuzumab resistance, tRNA-derived fragments, Physiology, QP1-981, Biochemistry, QD415-436
Abstract

Background/Aims: Resistance to trastuzumab remains a common challenge to HER-2 positive breast cancer. Up until now, the underlying mechanism of trastuzumab resistance is still unclear. tRNA-derived small non-coding RNAs, a new class of small non-coding RNA (sncRNAs), have been observed to play an important role in cancer progression. However, the relationship between tRNA-derived fragments and trastuzumab resistance is still unknown. Methods: We detected the levels of tRNA-derived fragments expression in normal breast epithelial cell lines, trastuzumab-sensitive and -resistant breast cancer cell lines using high-throughput sequencing. qRT-PCR was conducted to validate the differentially expressed fragments in serums from trastuzumab-sensitive and -resistant patients. A receiver operating characteristic (ROC) curve analysis was performed to evaluate the power of specific tRNA-derived fragments. Progression-free survival (PFS) was analyzed using Cox-regression. Results: Our sequence results showed that tRNA-derived fragments were differentially expressed in the HBL-100, SKBR3, and JIMT-1 cell lines. tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN were found significantly upregulated in trastuzumab-resistant patients compared to sensitive individuals, and the ROC analysis showed that tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN were correlated with trastuzumab resistance. In a multivariate analysis, higher levels of tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN expression were associated with significantly shorter PFS in patients with metastatic HER-2 positive breast cancer. Conclusion: Our results suggest that tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN play important roles in trastuzumab resistance. Patients with high levels of tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN expression benefitted less from trastuzumab-based therapy than those that express lower-levels of these molecules. tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN may be potential biomarkers and intervention targets in the clinical treatment of trastuzumab-resistant breast cancer.

Published
2018
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2. tRNA-Derived Fragments as Novel Predictive Biomarkers for Trastuzumab-Resistant Breast Cancer

Author

Jiahui Chu, Hao Wu, Yongfei Li, Jun Li, Ziyi Fu, Yanqiu Zhang, Yongmei Yin, Jian Wang, Ruihua Fan, Wei Li, Fan Yang, Yanhong Zhang, Xiang Huang, Zefei Jiang, Yifan Wang, and Chunxiao Sun

Subjects
0301 basic medicine, Physiology, Cell Survival, Receptor, ErbB-2, Breast Neoplasms, Disease-Free Survival, lcsh:Physiology, lcsh:Biochemistry, 03 medical and health sciences, Breast cancer, Antineoplastic Agents, Immunological, Downregulation and upregulation, RNA, Transfer, Trastuzumab, Cell Line, Tumor, medicine, Biomarkers, Tumor, Humans, lcsh:QD415-436, Neoplasm Metastasis, skin and connective tissue diseases, Trastuzumab resistance, tRNA-derived fragments, Proportional Hazards Models, Receiver operating characteristic, lcsh:QP1-981, business.industry, Cancer, RNA, medicine.disease, Prognosis, Gene Expression Regulation, Neoplastic, Survival Rate, 030104 developmental biology, ROC Curve, SKBR3, Cell culture, Drug Resistance, Neoplasm, Area Under Curve, Cancer research, Nucleic Acid Conformation, Female, business, medicine.drug
Abstract

Background/Aims: Resistance to trastuzumab remains a common challenge to HER-2 positive breast cancer. Up until now, the underlying mechanism of trastuzumab resistance is still unclear. tRNA-derived small non-coding RNAs, a new class of small non-coding RNA (sncRNAs), have been observed to play an important role in cancer progression. However, the relationship between tRNA-derived fragments and trastuzumab resistance is still unknown. Methods: We detected the levels of tRNA-derived fragments expression in normal breast epithelial cell lines, trastuzumab-sensitive and -resistant breast cancer cell lines using high-throughput sequencing. qRT-PCR was conducted to validate the differentially expressed fragments in serums from trastuzumab-sensitive and -resistant patients. A receiver operating characteristic (ROC) curve analysis was performed to evaluate the power of specific tRNA-derived fragments. Progression-free survival (PFS) was analyzed using Cox-regression. Results: Our sequence results showed that tRNA-derived fragments were differentially expressed in the HBL-100, SKBR3, and JIMT-1 cell lines. tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN were found significantly upregulated in trastuzumab-resistant patients compared to sensitive individuals, and the ROC analysis showed that tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN were correlated with trastuzumab resistance. In a multivariate analysis, higher levels of tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN expression were associated with significantly shorter PFS in patients with metastatic HER-2 positive breast cancer. Conclusion: Our results suggest that tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN play important roles in trastuzumab resistance. Patients with high levels of tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN expression benefitted less from trastuzumab-based therapy than those that express lower-levels of these molecules. tRF-30-JZOYJE22RR33 and tRF-27-ZDXPHO53KSN may be potential biomarkers and intervention targets in the clinical treatment of trastuzumab-resistant breast cancer.

Published
2018

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