1. Molecular characterisation of multidrug-resistant Mycobacterium tuberculosis isolates from a high-burden tuberculosis state in Brazil
- Author
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E. R. Dalla Costa, Afranio Lineu Kritski, I. N. de Almeida, I. S. Anusca, B. M. Praetzel, Gisela Unis, Sun Hee Schiefelbein, Regina Bones Barcellos, L. J. de Assis Figueredo, R. S. Salvato, Maria Laura Halon, Silvana Spíndola de Miranda, Leonardo Souza Esteves, Claudia Fontoura Dias, Maria Lucia Rosa Rossetti, and Elisangela C. Silva
- Subjects
0301 basic medicine ,Adult ,Male ,Tuberculosis ,Adolescent ,Databases, Factual ,Genotype ,Epidemiology ,tuberculosis (TB) ,030106 microbiology ,Antitubercular Agents ,Drug resistance ,Microbial Sensitivity Tests ,Minisatellite Repeats ,Biology ,Risk Assessment ,Mycobacterium tuberculosis ,03 medical and health sciences ,Young Adult ,Age Distribution ,Bacterial Proteins ,Cost of Illness ,Drug Resistance, Multiple, Bacterial ,Tuberculosis, Multidrug-Resistant ,medicine ,Humans ,Multidrug-Resistant Mycobacterium tuberculosis ,Sex Distribution ,Genotyping ,Retrospective Studies ,Original Paper ,drug resistance ,INHA ,Incidence ,DNA-Directed RNA Polymerases ,Middle Aged ,rpoB ,medicine.disease ,biology.organism_classification ,bacterial infections and mycoses ,Virology ,030104 developmental biology ,Infectious Diseases ,genotyping ,Female ,Rifampicin ,Brazil ,medicine.drug - Abstract
Tuberculosis (TB) is the leading cause of death among infectious diseases worldwide. Among the estimated cases of drug-resistant TB, approximately 60% occur in the BRICS countries (Brazil, Russia, India, China and South Africa). Among Brazilian states, primary and acquired multidrug-resistant TB (MDR-TB) rates were the highest in Rio Grande do Sul (RS). This study aimed to perform molecular characterisation of MDR-TB in the State of RS, a high-burden Brazilian state. We performed molecular characterisation of MDR-TB cases in RS, defined by drug susceptibility testing, using 131Mycobacterium tuberculosis (M.tb)DNA samples from the Central Laboratory. We carried out MIRU-VNTR 24loci, spoligotyping, sequencing of thekatG,inhA andrpoB genes and RDRiosublineage identification. The most frequent families found were LAM (65.6%) and Haarlem (22.1%). RDRiodeletion was observed in 42 (32%) of theM.tbisolates. Among MDR-TB cases, eight (6.1%) did not present mutations in the studied genes. In 116 (88.5%)M.tbisolates, we found mutations associated with rifampicin (RIF) resistance inrpoB gene, and in 112 isolates (85.5%), we observed mutations related to isoniazid resistance inkatG andinhA genes. An insertion of 12 nucleotides (CCAGAACAACCC) at the 516 codon in therpoB gene, possibly responsible for a decreased interaction of RIF and RNA polymerase, was found in 19/131 of the isolates, belonging mostly to LAM and Haarlem families. These results enable a better understanding of the dynamics of transmission and evolution of MDR-TB in the region.
- Published
- 2019