1. Studies on the differentiation of retinal pigmented epithelium cells in culture.
- Author
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Chader GJ, Newsome DA, Bensinger RE, and Fletcher RT
- Subjects
- Animals, Catechol Oxidase metabolism, Cells, Cultured, Chick Embryo, Chromatography, Gel, Clone Cells, Dextrans, Embryo, Mammalian, Embryo, Nonmammalian, Epithelial Cells, Melanins metabolism, Pigmentation, Triiodothyronine pharmacology, Tritium, Tyrosine pharmacology, Tyrosine 3-Monooxygenase metabolism, Cell Differentiation, Retina cytology
- Abstract
Sephadex G-25 fractions of chick embryo extract affect the differentiation of cloned embryonic pigmented epithelial cells (PE) in culture. Both tyrosinase activity and amounts of melanin are markedly affected by the extract fractions. 5-Hydroxyindole decreased melanin accumulation in PE cells while alpha-methyl-p-tyrosine had no apparent effect on the process. Melanin disposal as well as biosynthesis may be important in controlling the accumulation of pigment in PE cells under culture conditions. Triiodothyronine greatly increased visible pigmentation and affected the morphology of cultured PE cells. more...
- Published
- 1975