This article investigates a peculiar pattern of subject case-marking in the Greek of southern Italy. Recent fieldwork with native speakers, coupled with the consultation of some written sources, reveals that, alongside prototypical nominative subjects, Italo-Greek also licenses accusative subjects, despite displaying a predominantly nominative-accusative alignment. Far from being random replacements within a highly attrited grammar, the distribution of these accusative subjects obeys specific structural principles, revealing similarities with historical attestations of the so-called “extended accusative” in early Indo-European. On the basis of these data, Italo-Greek is argued to be undergoing a progressive shift towards an active-stative alignment, a claim supported by additional evidence from auxiliary selection, adverb agreement and sentential word order.