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Aim: To genotype in terms of cagA and vacA status, H. pylori clinical strains, isolated from children and adults in the Greek population. Materials and Methods: 45 clinical strains from children and 56 from adult patients were isolated from gastric biopsies following gastroscopy. The presence of cagA as well as subtyping of vacA mid-and signal-region was effected by PCR on genomic DNA as described by Atherton et al, 1999 and Yamaoka et al., 1999. Results: 84 (83%) out of 101 strains were detected positive for the presence of cagA (86% in the adult and 80% in the children population). Out of 79 strains analysed, 52 belonged to the vacAs1 and 27 to the vacAs2 subtype (66% and 34% respectively). Out of 74 strains analysed, 23 (31%) were of the vacAm1 and 48 (65%) vacAm2 subtypes while 3 (4%) remained untypeable. The combinations of vacA subtypes observed are summarized in the following table: We did not detect the vacAs2/m1 subtype in the sample population. Conclusion: A high frequency of cagA positive strains was observed in the Greek population. There was no significant difference in the frequency between the vacA mid- and signal-region subtypes. No statistically significant differences among the subtypes were detected between the adult and the children study groups. [ABSTRACT FROM AUTHOR]