1. The effect of human platelet lysate on corneal nerve regeneration
- Author
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Kuo-Chi Hung, Mei-Yun Chen, Wei-Li Chen, Chin-Te Huang, Fung-Rong Hu, Hsiao-Sang Chu, and Lily Wei Chen
- Subjects
Adult ,Blood Platelets ,Male ,Cornea ,Rats, Sprague-Dawley ,Andrology ,Mice ,Cellular and Molecular Neuroscience ,Trigeminal ganglion ,In vivo ,Neurotrophic factors ,Animals ,Humans ,Medicine ,Nerve Growth Factors ,Cells, Cultured ,Cell Proliferation ,Wound Healing ,biology ,business.industry ,Regeneration (biology) ,Epithelium, Corneal ,Cell Differentiation ,Healthy Volunteers ,eye diseases ,Sensory Systems ,Nerve Regeneration ,Rats ,Disease Models, Animal ,Ophthalmology ,biology.protein ,Female ,Platelet lysate ,business ,Wound healing ,Fetal bovine serum ,Corneal Injuries ,Neurotrophin - Abstract
AimThis study aimed to test whether human platelet lysate (HPL) has neurotrophic ability for corneal nerve regeneration.MethodsWe measured the neurotrophic factors in human peripheral serum (HPS) and two commercially available HPLs, UltraGRO and PLTMax. In vitro, we compared the growth rates, neuronal differentiation and immunostaining of neuron markers in mouse neuroblastoma cell line (Neuro-2a) and primary culture of mouse trigeminal ganglion cells that were cultivated in different concentrations of fetal bovine serum, HPS and HPL. In vivo, we created corneal wounds on Sprague Dawley rats with a rotating burr and evaluated the effects of topical HPL on wound healing and corneal nerve regeneration by in vivo confocal microscopy and corneal aesthesiometry.ResultsHPLs had significantly higher concentrations of various neurotrophic factors compared with HPS (pConclusionHPL has significantly higher concentrations of neurotrophic factors compared with HPS. It showed not only in vitro but also in vivo corneal neurotrophic abilities. Our results suggest that HPL may have a potential role in the treatment of diseases related to corneal nerve damage or degeneration.
- Published
- 2019