1. New markers and an old phenomenon: prozone effect disturbing detection of filaggrin (keratin) autoantibodies
- Author
-
Damien Dubois, Gilles Renier, Frederique Dubois-Galopin, Pascale Jeannin, Charles Masson, Céline Beauvillain, Alain Chevailler, and Anne Pillet
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Immunology ,Filaggrin Proteins ,Biology ,Peptides, Cyclic ,Sensitivity and Specificity ,General Biochemistry, Genetics and Molecular Biology ,Arthritis, Rheumatoid ,Intermediate Filament Proteins ,Rheumatology ,Keratin ,medicine ,Stratum corneum ,Humans ,Immunology and Allergy ,Letters ,Fluorescent Antibody Technique, Indirect ,Aged ,Autoantibodies ,Aged, 80 and over ,chemistry.chemical_classification ,integumentary system ,Autoantibody ,IIf ,Middle Aged ,Epithelium ,Staining ,medicine.anatomical_structure ,chemistry ,biology.protein ,Female ,Antibody ,Biomarkers ,Filaggrin - Abstract
Anti-filaggrin antibodies, also named anti-keratin antibodies (AKA), are serum IgG labelling the stratum corneum of the rat oesophagus epithelium, detectable by indirect immunofluorescence (IIF). They constitute a specific marker for the diagnosis of rheumatoid arthritis (RA).1–3 A laminar staining pattern is seen with most AKA-positive sera (fig 1A), but occasionally the cornified layer is diffusely labelled, and the image also shows foamy staining (fig 1B). Although most authors consider sera as AKA positive only when a linear laminated pattern is observed,4,5 some consider the sera as AKA positive even when the staining is diffuse or foamy.6 Figure 1 Anti-keratin antibodies (AKA) detected by indirect immunofluorescence on rat oesophagus. (A) Typical linear laminated labelling pattern restricted to the upper cornified layer observed in the serum …
- Published
- 2007