1. Discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing (MPSS)
- Author
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Jian Huang, Yun-Li Zhang, Ze-Guang Han, Xuegong Zhang, Peng-Yuan Yang, Yun Wang, Qing Deng, Fu-Xing Deng, Yi Hong, Xiaowo Wang, Pei Hao, Yixue Li, Hong-Yang Wang, and Tingting Li
- Subjects
lcsh:QH426-470 ,lcsh:Biotechnology ,Amino Acid Motifs ,UniGene ,Genomics ,Computational biology ,Biology ,Proteomics ,Massively parallel signature sequencing ,lcsh:TP248.13-248.65 ,Genetics ,Humans ,RNA, Messenger ,Gene Library ,Oligonucleotide Array Sequence Analysis ,Laser capture microdissection ,Expressed Sequence Tags ,Expressed sequence tag ,Models, Statistical ,Models, Genetic ,Gene Expression Profiling ,Gene expression profiling ,lcsh:Genetics ,Gene Expression Regulation ,Genetic Techniques ,Liver ,Multigene Family ,Hepatocytes ,DNA microarray ,Research Article ,Biotechnology - Abstract
Background The liver is the largest human internal organ – it is composed of multiple cell types and plays a vital role in fulfilling the body's metabolic needs and maintaining homeostasis. Of these cell types the hepatocytes, which account for three-quarters of the liver's volume, perform its main functions. To discover the molecular basis of hepatocyte function, we employed Massively Parallel Signature Sequencing (MPSS) to determine the transcriptomic profile of adult human hepatocytes obtained by laser capture microdissection (LCM). Results 10,279 UniGene clusters, representing 7,475 known genes, were detected in human hepatocytes. In addition, 1,819 unique MPSS signatures matching the antisense strand of 1,605 non-redundant UniGene clusters (such as APOC1, APOC2, APOB and APOH) were highly expressed in hepatocytes. Conclusion Apart from a large number of protein-coding genes, some of the antisense transcripts expressed in hepatocytes could play important roles in transcriptional interference via a cis-/trans-regulation mechanism. Our result provided a comprehensively transcriptomic atlas of human hepatocytes using MPSS technique, which could be served as an available resource for an in-depth understanding of human liver biology and diseases.
- Published
- 2007