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2 results on '"Shuyu Hu"'

1. All-trans retinoic acid stimulates the secretion of TGF-β2 via the phospholipase C but not the adenylyl cyclase signaling pathway in retinal pigment epithelium cells

Author

Daren Zhang, Zhihong Deng, Jia Tan, Shuirong Liu, Shuyu Hu, Hui Tao, and Renhong Tang

Subjects
Transforming growth factor-β2, U73122, SQ22536, Retinal pigment epithelium cells, All-trans retinoic acid, Ophthalmology, RE1-994
Abstract

Abstract Background By investigating that (i) all-trans retinoic acid (ATRA) affects human retinal pigment epithelium (RPE) in expressing and secreting transforming growth factor (TGF)-β2 and (ii) U73122 (phospholipase C inhibitor) and SQ22536 (adenylyl cyclase inhibitor) regulate the ATRA-induced secretion of TGF-β2 in human RPE, we sought to interpret the signaling pathway of ATRA in promoting the development of myopia. Methods The RPE cell line (D407) was treated with (i) ATRA (10 μM), (ii) U73122 (5–40 μM) and ATRA (10 μM), or (iii) SQ22536 (5–40 μM) and ATRA (10 μM). The control group was no-treated. After stimulated at 2, 4, 8, 16, 24, and 48 h, The expression and secretion of TGF-β2 was detected. Results TGF-β2 in the cytoplasm was time-dependent increased by ATRA (p 0.05). Conclusions In RPE cells, ATRA stimulates the secretion of TGF-β2 via the phospholipase C signaling pathway but not the adenylyl cyclase signaling pathway. U73122 may inhibit the promotion of ATRA in the development of myopia.

Published
2019
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2. All-trans retinoic acid stimulates the secretion of TGF-β2 via the phospholipase C but not the adenylyl cyclase signaling pathway in retinal pigment epithelium cells

Author

Hui Tao, Shuirong Liu, Ren-hong Tang, Daren Zhang, Shuyu Hu, Jia Tan, and Zhihong Deng

Subjects
Cytoplasm, Retinoic acid, Tretinoin, Retinal Pigment Epithelium, Adenylyl cyclase, Transforming Growth Factor beta2, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, lcsh:Ophthalmology, SQ22536, Myopia, Humans, Medicine, Secretion, neoplasms, Cells, Cultured, Retinal pigment epithelium, Phospholipase C, business.industry, All-trans retinoic acid, organic chemicals, Epithelial Cells, General Medicine, U73122, Retinal pigment epithelium cells, Phospholipase C Signaling Pathway, biological factors, Up-Regulation, Cell biology, Ophthalmology, medicine.anatomical_structure, chemistry, lcsh:RE1-994, Type C Phospholipases, 030221 ophthalmology & optometry, Transforming growth factor-β2, sense organs, Signal transduction, business, 030217 neurology & neurosurgery, Adenylyl Cyclases, Signal Transduction, Research Article, Transforming growth factor
Abstract

Background By investigating that (i) all-trans retinoic acid (ATRA) affects human retinal pigment epithelium (RPE) in expressing and secreting transforming growth factor (TGF)-β2 and (ii) U73122 (phospholipase C inhibitor) and SQ22536 (adenylyl cyclase inhibitor) regulate the ATRA-induced secretion of TGF-β2 in human RPE, we sought to interpret the signaling pathway of ATRA in promoting the development of myopia. Methods The RPE cell line (D407) was treated with (i) ATRA (10 μM), (ii) U73122 (5–40 μM) and ATRA (10 μM), or (iii) SQ22536 (5–40 μM) and ATRA (10 μM). The control group was no-treated. After stimulated at 2, 4, 8, 16, 24, and 48 h, The expression and secretion of TGF-β2 was detected. Results TGF-β2 in the cytoplasm was time-dependent increased by ATRA (p 0.05). Conclusions In RPE cells, ATRA stimulates the secretion of TGF-β2 via the phospholipase C signaling pathway but not the adenylyl cyclase signaling pathway. U73122 may inhibit the promotion of ATRA in the development of myopia.

Published
2019
Full Text
View/download PDF

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