Search

Your search keyword '"Robert J. Kimmerling"' showing total 2 results
Start Over Author "Robert J. Kimmerling" Publisher bmc
2 results on '"Robert J. Kimmerling"'

1. Linking single-cell measurements of mass, growth rate, and gene expression

Author

Robert J. Kimmerling, Sanjay M. Prakadan, Alejandro J. Gupta, Nicholas L. Calistri, Mark M. Stevens, Selim Olcum, Nathan Cermak, Riley S. Drake, Kristine Pelton, Frederik De Smet, Keith L. Ligon, Alex K. Shalek, and Scott R. Manalis

Subjects
Single-cell RNA-Seq, Mass, Growth, Serial suspended microchannel resonator, Multi-omics, Single cell, Biology (General), QH301-705.5, Genetics, QH426-470
Abstract

Abstract Mass and growth rate are highly integrative measures of cell physiology not discernable via genomic measurements. Here, we introduce a microfluidic platform enabling direct measurement of single-cell mass and growth rate upstream of highly multiplexed single-cell profiling such as single-cell RNA sequencing. We resolve transcriptional signatures associated with single-cell mass and growth rate in L1210 and FL5.12 cell lines and activated CD8+ T cells. Further, we demonstrate a framework using these linked measurements to characterize biophysical heterogeneity in a patient-derived glioblastoma cell line with and without drug treatment. Our results highlight the value of coupled phenotypic metrics in guiding single-cell genomics.

Published
2018
Full Text
View/download PDF

2. Linking single-cell measurements of mass, growth rate, and gene expression

Author

Alex K. Shalek, Riley S. Drake, Nicholas L. Calistri, Keith L. Ligon, Selim Olcum, Alejandro J. Gupta, Scott R. Manalis, Robert J. Kimmerling, Kristine Pelton, Sanjay Prakadan, Mark M. Stevens, Frederik De Smet, Nathan Cermak, Institute for Medical Engineering and Science, Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Chemistry, Koch Institute for Integrative Cancer Research at MIT, Kimmerling, Robert John, Prakadan, Sanjay, Gupta, Alejandro J., Calistri, Nicholas L, Stevens, Mark M., Olcum, Selim A., Cermak, Nathan, Drake, Riley, Shalek, Alexander K, and Manalis, Scott R

Subjects
0301 basic medicine, Cell, Microfluidics, Method, Growth, CD8-Positive T-Lymphocytes, Lymphocyte Activation, Mice, Gene expression, Single cell, HETEROGENEITY, lcsh:QH301-705.5, Genetics & Heredity, Multi-omics, T cell activation, Genomics, Microfluidic Analytical Techniques, Biophysical properties, Phenotype, GENOME, medicine.anatomical_structure, DIFFERENTIATION, TARGET, Single-Cell Analysis, Life Sciences & Biomedicine, Single-cell RNA-Seq, Cell physiology, lcsh:QH426-470, Serial suspended microchannel resonator, Drug response, Computational biology, Cell Enlargement, Biology, METABOLISM, GBM, 03 medical and health sciences, Cell Line, Tumor, medicine, Animals, Humans, Science & Technology, RNA, Mass, lcsh:Genetics, 030104 developmental biology, lcsh:Biology (General), Biotechnology & Applied Microbiology, Cell culture, TISSUE, Glioblastoma, CD8
Abstract

Mass and growth rate are highly integrative measures of cell physiology not discernable via genomic measurements. Here, we introduce a microfluidic platform enabling direct measurement of single-cell mass and growth rate upstream of highly multiplexed single-cell profiling such as single-cell RNA sequencing. We resolve transcriptional signatures associated with single-cell mass and growth rate in L1210 and FL5.12 cell lines and activated CD8+ T cells. Further, we demonstrate a framework using these linked measurements to characterize biophysical heterogeneity in a patient-derived glioblastoma cell line with and without drug treatment. Our results highlight the value of coupled phenotypic metrics in guiding single-cell genomics. Keywords: Single-cell RNA-Seq, Mass, Growth, Serial suspended microchannel resonator, Multi-omics, Single cell, T cell activation, Glioblastoma, GBM, Drug response, Microfluidics, Biophysical properties

Published
2018

Catalog

Books, media, physical & digital resources
See catalog results