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3 results on '"Dongxia Ye"'

1. Activation of mesenchymal stem cells promotes new bone formation within dentigerous cyst

Author

Yejia Yu, Mengyu Li, Yuqiong Zhou, Yueqi Shi, Wenjie Zhang, Geehun Son, Jing Ge, Jun Zhao, Zhiyuan Zhang, Dongxia Ye, Chi Yang, and Shaoyi Wang

Subjects
Dentigerous cyst, Mesenchymal stem cells, Osteogenic differentiation, Bone regeneration, Cell proliferation, Cell culture, Medicine (General), R5-920, Biochemistry, QD415-436
Abstract

Abstract Background Dentigerous cyst (DC) is a bone destructive disease and remains a challenge for clinicians. Marsupialization enables the bone to regenerate with capsule maintaining, making it a preferred therapeutic means for DC adjacent to vital anatomical structures. Given that capsules of DC are derived from odontogenic epithelium remnants at the embryonic stage, we investigated whether there were mesenchymal stem cells (MSCs) located in DC capsules and the role that they played in the bone regeneration after marsupialization. Methods Samples obtained before and after marsupialization were used for histological detection and cell culture. The stemness of cells isolated from fresh tissues was analyzed by morphology, surface marker, and multi-differentiation assays. Comparison of proliferation ability between MSCs isolated from DC capsules before (Bm-DCSCs) and after (Am-DCSCs) marsupialization was evaluated by Cell Counting Kit-8 (CCK-8), fibroblast colony-forming units (CFU-F), and 5′-ethynyl-2′-deoxyuridine (EdU) assay. Their osteogenic capacity in vitro was detected by alkaline phosphatase (ALP) and Alizarin Red staining (ARS), combined with real-time polymerase chain reaction (RT-PCR) and immunofluorescence (IF) staining. Subcutaneous ectopic osteogenesis as well as cranial bone defect model in nude mice was performed to detect their bone regeneration and bone defect repairability. Results Bone tissue and strong ALP activity were detected in the capsule of DC after marsupialization. Two types of MSCs were isolated from fibrous capsules of DC both before (Bm-DCSCs) and after (Am-DCSCs) marsupialization. These fibroblast-like, colony-forming cells expressed MSC markers (CD44+, CD90+, CD31−, CD34−, CD45−), and they could differentiate into osteoblast-, adipocyte-, and chondrocyte-like cells under induction. Notably, Am-DCSCs performed better in cell proliferation and self-renewal. Moreover, Am-DCSCs showed a greater osteogenic capacity both in vitro and in vivo compared with Bm-DCSCs. Conclusions There are MSCs residing in capsules of DC, and the cell viability as well as the osteogenic capacity of them is largely enhanced after marsupialization. Our findings suggested that MSCs might play a crucial role in the healing process of DC after marsupialization, thus providing new insight into the treatment for DC by promoting the osteogenic differentiation of MSCs inside capsules.

Published
2020
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2. Role of toll-like receptor 4 on the immune escape of human oral squamous cell carcinoma and resistance of cisplatin-induced apoptosis

Author

Wantao Chen, Qingqiong Luo, Zujun Sun, Fuxiang Chen, and Dongxia Ye

Subjects
Lipopolysaccharides, Vascular Endothelial Growth Factor A, Cancer Research, Apoptosis, Lipopolysaccharide, Biology, p38 Mitogen-Activated Protein Kinases, lcsh:RC254-282, Immune system, Cell Line, Tumor, medicine, Humans, Receptor, Cisplatin, Toll-like receptor, Interleukin-6, Research, Interleukin-8, NF-kappa B, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Toll-like receptors, Gene Expression Regulation, Neoplastic, Toll-Like Receptor 4, stomatognathic diseases, Oncology, Oral squamous cell carcinoma, Cell culture, Myeloid Differentiation Factor 88, Cancer research, TLR4, Carcinoma, Squamous Cell, Molecular Medicine, Mouth Neoplasms, RNA Interference, Tumor Escape, Myeloid differentiation primary response gene 88, Signal transduction, medicine.drug, Signal Transduction
Abstract

Background Toll-like receptor 4 (TLR4) is expressed on immune cells as a sensor that recognizes lipopolysaccharide (LPS), a microbial conserved component. It has recently been determined that the expression of TLR4 is also found in various types of tumor cells. Cisplatin is a widely used chemotherapeutic agent for oral squamous cell carcinoma (OSCC) treatment. However, the mechanisms responsible for cisplatin resistance are not well understood. Results The present study was designed to elucidate the role of TLR4 expression in human OSCC regarding immune escape and apoptotic resistance to cisplatin. TLR4 and the myeloid differentiation primary response gene 88 (MyD88) were highly expressed in OSCC cell lines. Upon LPS stimulation both NF-κB and p38 MAPK pathways were activated in OSCC cell lines, followed by the production of large quantities of IL-6, IL-8 and VEGF compared with human immortalized oral epithelia cells (HIOECs). OSCC cell lines were found to be resistant to cisplatin-mediated apoptosis after pretreatment with LPS. Conclusions Our results suggested that TLR4 was functionally expressed in human OSCC cells and development of resistance to cisplatin in human OSCC might occur through the mechanism involving TLR4 and its signaling pathway. Suppression of TLR4 and its signaling pathway might thus elevate sensitivity to cisplatin and potentially help improve the prognosis of patients with OSCC.

Published
2012

3. Response of lymphocyte subsets and cytokines to Shenyang prescription in Sprague-Dawley rats with tongue squamous cell carcinomas induced by 4NQO

Author

Wantao Chen, Di He, Dongxia Ye, Xiuli Zhang, Zhiyuan Zhang, Can-hua Jiang, Ping Zhang, and Weiliu Qiu

Subjects
Male, Pathology, medicine.medical_specialty, Cancer Research, Cell, Drug Evaluation, Preclinical, Antineoplastic Agents, Cell Count, lcsh:RC254-282, Natural killer cell, Rats, Sprague-Dawley, T-Lymphocyte Subsets, Immunity, Surgical oncology, Tongue, medicine, Genetics, Animals, Medicine, Chinese Traditional, business.industry, Cancer, T-Lymphocytes, Helper-Inducer, medicine.disease, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, 4-Nitroquinoline-1-oxide, Rats, Tongue Neoplasms, medicine.anatomical_structure, Oncology, Immunology, Carcinoma, Squamous Cell, Cytokines, Immunocompetence, Stem cell, business, Drugs, Chinese Herbal, Research Article
Abstract

BackgroundThe study was designed to investigate immunocompetence in relation to cancer progression in rat and to assess the effect of the traditional Chinese anti-cancer medicine, "Shenyang" prescription, on immunity.Methods4-Nitroquinoline-1-oxide (4NQO) was administered to 80 Sprague-Dawley (SD) rats via the drinking water for up to 36 weeks. Tongue squamous cell carcinoma (SCC) was confirmed by pathological examination in 61 rats. "Shenyang" prescription was administered to subgroups of these rats, and blood samples were taken before and after treatment. Lymphocyte subsets were determined by flow cytometry. Serum Th1 and Th2-type cytokines were assessed by an enzyme-linked immunosorbent assay.ResultsAs the cancer progressed at the tongue root, the percentage of CD3+CD4+ T lymphocytes and NK cells and the levels of IFN-γ and IL-2 decreased gradually, while the percentage of CD3+CD8+ T lymphocytes and the levels of IL-4 and IL-10 increased. The CD4+/CD8+ ratios were lower in the cancer groups than in the control group. However, after administering "Shenyang" prescription, the levels of CD3+CD4+ T lymphocytes, NK cells, IFN-γ and IL-2 increased, while the CD3+CD8+ T lymphocyte counts and the levels of IL-4 and IL-10 decreased.Conclusion4NQO-induced lesions were good models for exploring oral cavity carcinogenesis. The rats with 4NQO-induced SCC demonstrated abnormalities in lymphocyte subsets and a shift from Th1-type to Th2-type, which were good models for assessing the effect of anticancer agent on immunity. Oral cancer progression was associated with an aggressive disturbance of immune function. "Shenyang" prescription has the ability to improve the disturbance of immune function.

Published
2007

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