1. Semi-quantitative duplex RT-PCR reveals the low occurrence of Porcine Pegivirus and Atypical Porcine Pestivirus in diagnostic samples from the United States.
- Author
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Chen F, Knutson TP, Braun E, Jiang Y, Rossow S, and Marthaler DG
- Subjects
- Animals, Flaviviridae genetics, Flaviviridae Infections epidemiology, Flaviviridae Infections virology, Minnesota epidemiology, Pestivirus genetics, Pestivirus isolation & purification, Pestivirus Infections epidemiology, Pestivirus Infections veterinary, Pestivirus Infections virology, Phylogeny, Reverse Transcriptase Polymerase Chain Reaction veterinary, Swine, Swine Diseases virology, United States epidemiology, Coinfection veterinary, Flaviviridae isolation & purification, Flaviviridae Infections veterinary, Metagenomics, Swine Diseases epidemiology
- Abstract
Porcine Pegivirus (PPgV) and Atypical Porcine Pestivirus (APPV) are two recently identified porcine viruses. In this study, the identification of two viruses by metagenomic sequencing, and a duplex semi-quantitative RT-PCR was developed to detect these pathogens simultaneously. The PPgV strain Minnesota-1/2016 had a 95.5%-96.3% nucleotide identity and clustered with the recently identified US PPgV strains, which is a distant clade from the German PPgV strains. The APPV strain Minnesota-1/2016 shared an 87.3%-92.0% nucleotide identity with the other global APPV strains identity but only shared an 82.8%-83.0% nucleotide identity with clade II consisting of strain identified in China. Detection of both PPgV and APPV was 9.0% of the diagnostic cases. Co-infection of PPgV and APPV was identified in 7.5% of the diagnostic cases. The occurrence and genetic characterization of PPgV and APPV further enhance our knowledge regarding these new pathogens in the United States., (© 2019 The Authors. Transboundary and Emerging Diseases published by Blackwell Verlag GmbH.)
- Published
- 2019
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