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Your search keyword '"R. Wernery"' showing total 2 results
Start Over Author "R. Wernery" Publisher blackwell verlag
2 results on '"R. Wernery"'

1. Genomic tandem repeat analysis proves laboratory-acquired brucellosis in veterinary (camel) diagnostic laboratory in the United Arab Emirates.

Author

Schulze zur Wiesch J, Wichmann D, Sobottka I, Rohde H, Schmoock G, Wernery R, Schmiedel S, Dieter Burchard G, and Melzer F

Subjects
Animals, Anti-Bacterial Agents therapeutic use, Brucellosis drug therapy, Brucellosis epidemiology, Brucellosis microbiology, Humans, Male, Middle Aged, Milk microbiology, Nucleic Acid Hybridization, United Arab Emirates epidemiology, Zoonoses transmission, Brucellosis veterinary, Camelus, Tandem Repeat Sequences genetics, Zoonoses epidemiology
Abstract

We report a case of a 64-year-old veterinarian working in a state camel veterinary laboratory who was diagnosed with and treated for acute brucellosis with complicating epididymo-orchitis. Genomic tandem repeat analysis (MLVA-16) revealed identical Brucella strains in patient cultures and from different dromedary milk samples positive for Brucella melitensis, thereby confirming the diagnosis of a laboratory acquired infection. The case illustrates the high (airborne) infectivity of brucellosis in laboratory settings and the need to implement vigorous bio-safety measures in veterinary laboratories handling camel specimen diagnostic veterinary laboratory.

Published
2010
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2. Development and clinical evaluation of a PCR assay targeting the metalloprotease gene (mprA) of B. pseudomallei.

Author

Neubauer H, Sprague LD, Joseph M, Tomaso H, Al Dahouk S, Witte A, Kinne J, Hensel A, Wernery R, Wernery U, and Scholz HC

Subjects
Animals, Base Sequence, DNA Primers, DNA, Bacterial chemistry, DNA, Bacterial genetics, Gene Amplification, Humans, Molecular Sequence Data, Polymerase Chain Reaction methods, Sensitivity and Specificity, Species Specificity, Burkholderia pseudomallei isolation & purification, Metalloproteases genetics, Polymerase Chain Reaction veterinary
Abstract

A PCR assay targeting the metalloprotease gene (mprA) of Burkholderia pseudomallei was developed for the specific detection of this organism in pure cultures and clinical samples. All other closely related organisms including B. mallei the causative agent of glanders, and B. thailandensis tested negative. Burkholderia pseudomallei DNA was successfully amplified from paraffin-embedded lung tissue of a camel with a generalized B. pseudomallei infection. The developed PCR assay can be used as a simple tool for the specific and sensitive detection of B. pseudomallei.

Published
2007
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