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Anaplasma species are obligate intracellular rickettsial pathogens transmitted by ticks with an impact on human and animal health. Anaplasma ovis infects sheep and goats in many regions of the world, and it can be diagnosed by different methods like Giemsa staining, PCR or competitive ELISA. In this study, a PCR based on the gene coding for major surface protein 4 (MSP-4) was used to examine field samples collected from sheep in different countries. Altogether, 1161 blood samples from Turkey (n = 830), Iraq (n = 195), Sudan (n = 96) and Portugal (n = 40) were examined, of which 31.4%, 66.6% 41.6% and 82.5%, respectively, were positive. This indicates high prevalence of A. ovis in the countries under investigation, and it can be assumed that the situation in other areas of the world might be similar. Thus, A. ovis should be considered as an important constraint of livestock production, and further efforts are needed to better understand the epidemiology and to implement suitable control measures., (© 2013 Blackwell Verlag GmbH.)

Quality and safe meat production and livestock husbandry are important foci for addressing the wider underlying economic and political challenges. In the last few years, an intense focus of the scientific community has been placed on breakouts of livestock diseases especially in Asia, which have spread into neighbouring countries including Europe. These outbreaks had a serious impact on the livelihood of the farmers as well as the economy of the affected countries. Given this, the establishment of a network of diagnostic facilities is a great demand both at the national and regional levels. In most of the cases, diagnostic assays are either not available or they are not validated. The aim of this collaborative network was to: 1 Distribute and harmonize diagnostic tools required for pathogen detection and differentiation. 2 Build the capacity to ensure the conduction of integrated disease control measures.