1. Sphingosylphosphorylcholine activates dendritic cells, stimulating the production of interleukin-12.
- Author
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Ceballos A, Sabatté J, Nahmod K, Martínez D, Salamone G, Vermeulen M, Maggini J, Salomón H, and Geffner J
- Subjects
- Antigens, CD metabolism, B7-2 Antigen metabolism, Calcium metabolism, Cells, Cultured, Chemotaxis immunology, Dendritic Cells metabolism, Endocytosis immunology, HLA-DR Antigens metabolism, Humans, Immunoglobulins metabolism, Interferon-gamma biosynthesis, Interleukin-18 biosynthesis, Lymphocyte Culture Test, Mixed, Membrane Glycoproteins metabolism, Phosphorylcholine immunology, Receptors, Lysosphingolipid metabolism, Reverse Transcriptase Polymerase Chain Reaction methods, Sphingosine immunology, CD83 Antigen, Dendritic Cells immunology, Interleukin-12 biosynthesis, Phosphorylcholine analogs & derivatives, Sphingosine analogs & derivatives
- Abstract
Compared with other lysophospholipid mediators such as sphingosine-1-phosphate and lysophosphatidic acid, little is known about the physiological significance of the related bioactive lysosphingolipid sphingosylphosphorylcholine (SPC), which is present in high-density lipoprotein particles. The present study was undertaken to evaluate the effect of SPC on human immature dendritic cells (DCs). Reverse transcription-polymerase chain reaction and flow cytometry assays revealed that DCs express two putative receptors for SPC, ovarian cancer G-protein-coupled receptor 1 and G-protein-coupled receptor 4. Exposure to SPC induced a rapid and transient increase in intracellular free calcium concentrations but did not stimulate endocytosis or chemotaxis of DCs. SPC increased the expression of HLA-DR, CD86 and CD83 and improved the T-cell priming ability of DCs, as well as the ability of DCs to stimulate the production of interferon-gamma by allogeneic peripheral blood mononuclear cells during the mixed lymphocyte reaction. Consistent with these results, we also observed that SPC stimulated the production of interleukin (IL)-12 and IL-18 by DCs. Taken together, our results support the notion that the accumulation of SPC in peripheral tissues during the course of inflammatory processes may favour the development of T helper type 1 immunity.
- Published
- 2007
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