1. Studies on Clostridium acetobutylicum glnA promoters and antisense RNA.
- Author
-
Janssen PJ, Jones DT, and Woods DR
- Subjects
- Base Sequence, Clostridium enzymology, Escherichia coli genetics, Genes, Bacterial, Glutamate-Ammonia Ligase metabolism, Molecular Sequence Data, Mutation, Nitrogen pharmacology, RNA, Antisense metabolism, Repetitive Sequences, Nucleic Acid, Transcription, Genetic, Clostridium genetics, Gene Expression Regulation, Bacterial, Glutamate-Ammonia Ligase genetics, Promoter Regions, Genetic, RNA, Antisense genetics
- Abstract
The Clostridium acetobutylicum glnA gene has two transcript start sites under the control of promoters p1 and p2. Initiation of transcription was regulated by nitrogen and a downstream region was implicated in the regulation of transcript initiation by nitrogen in Escherichia coli. Putative antisense RNA was produced from a single downstream transcript start site under the control of p3. An up-promoter mutation in p3 resulted in lower levels of glutamine synthetase (GS) activity. Putative antisense RNA had a role in down-regulating GS expression but was not involved in regulation by nitrogen. Deletion of downstream inverted repeat sequences resulted in very low levels of GS activity.
- Published
- 1990