1. B cell-activating factor enhances interleukin-6 and interleukin-10 production by ODN-activated human B cells.
- Author
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Yehudai D, Snir A, Peri R, Halasz K, Haj T, Odeh M, and Kessel A
- Subjects
- Antigens, CD genetics, Antigens, CD metabolism, Antigens, Differentiation, T-Lymphocyte genetics, Antigens, Differentiation, T-Lymphocyte metabolism, B-Cell Activating Factor immunology, B-Lymphocytes cytology, B-Lymphocytes drug effects, Cell Differentiation drug effects, CpG Islands genetics, CpG Islands immunology, Drug Interactions, Gene Expression Regulation drug effects, Histocompatibility Antigens Class II genetics, Histocompatibility Antigens Class II metabolism, Humans, Interleukin-10 immunology, Interleukin-6 immunology, Lectins, C-Type genetics, Lectins, C-Type metabolism, Lymphocyte Activation drug effects, Oligodeoxyribonucleotides immunology, Primary Cell Culture, Signal Transduction drug effects, Signal Transduction genetics, Toll-Like Receptor 9 genetics, B-Cell Activating Factor pharmacology, B-Lymphocytes metabolism, Interleukin-10 biosynthesis, Interleukin-6 biosynthesis, Oligodeoxyribonucleotides pharmacology, Toll-Like Receptor 9 metabolism
- Abstract
We aim to investigate the additive value of B cell-activating factor (BAFF) when added to oligodeoxynucleotides (ODN)-activated B cells with respect to TLR-9, CD69, MHC-II expression, IL-6 and IL-10 secretion and B cell cycling. Therefore, B cells from healthy individuals were incubated under the following conditions: (1) B cells with medium, (2) B cells with ODN 0.5 μm, (3) B cells with BAFF 20 μm and (4) B cells with both ODN 0.5 μm and BAFF 20 μm. We found that addition of BAFF did not enhance the expression of TLR-9, CD69 and MHC-II in ODN-activated B cells. Incubation of B cells with BAFF and ODN together leads to a marked elevation of IL-6 and IL-10 levels compared to ODN alone. Synthesis and mitosis were higher in B cells stimulated by BAFF than in B cells stimulated by ODN. These findings suggest that both BAFF and TLR-9 contribute independently to B cell function., (© 2012 The Authors. Scandinavian Journal of Immunology © 2012 Blackwell Publishing Ltd.)
- Published
- 2012
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