1. Microsomal 12alpha-hydroxylation of 7alpha-[12alpha, 12beta-2H2]hydroxy-4-cholesten-3-one.
- Author
-
Björkhem I
- Subjects
- Animals, Deuterium, Hydroxylation, Hydroxysteroids chemical synthesis, Hydroxysteroids metabolism, Kinetics, Male, Rats, Cholestenes, Cholestenones chemical synthesis, Cholestenones metabolism, Microsomes, Liver metabolism
- Abstract
The synthesis of 7alpha-[12alpha, 12beta-2 H2]hydroxy-4-cholesten-3-one is described. It was shown with different techniques that this compound was 12alpha-hydroxylated by the microsomal fraction of a rat liver homogenate without marked isotope effect, indicating that cleavage of the C--H bond is not the rate-limiting step in this hydroxylation. The rate of 12alpha-hydroxylation was decreased by about 20% when performed in a medium containing deuterated water. The findings were discussed with reference to the specific properties of the 12alpha-hydroxylating system and to the results of previous studies on rate-limiting step in microsomal hydroxylation of steroids.
- Published
- 1975
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